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From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

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Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy...
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Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

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Light sheet-based fluorescence microscopy is the most valuable tool in developmental biology. A major issue in comparative studies is ambient variance. Our protocol describes an experimental framework for simultaneous live imaging of multiple specimens and, therefore, addresses this issue...
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Live Cell Imaging of Chromosome Segregation During Mitosis

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This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy...
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High-Resolution C. elegans Imaging Across All Larval Stages07:49

High-Resolution C. elegans Imaging Across All Larval Stages

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Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function

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Presented here is a protocol using a temperature-controlled microscope stage that allows a sample container to be mounted on a vertical...
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Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

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The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain...
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相关实验视频

Updated: Jan 20, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.9K

微拉伸器 (MicroStretch):用于在微观阶段进行实时成像的微拉伸器.

Alexi Switz1, Anamika Prasad1,2

  • 1Biomedical Engineering, Florida International University, Miami, FL, United States.

HardwareX
|January 19, 2026
PubMed
概括

研究人员开发了一种低成本的微型可编程系统,用于材料变形测试. 这种设备可以在微观阶段进行专门的拉伸测试,增强生物力学和材料科学研究.

科学领域:

  • 材料科学 材料科学 材料科学
  • 生物力学 生物力学
  • 工程 工程师 工程师 工程师

背景情况:

  • 材料变形评估对于理解各种领域的材料行为至关重要.
  • 现有的商业测试机器往往是大型,昂贵和沉重的,限制了专门的应用.
  • 微型化对于在微观或光谱阶段测试软和生物医学材料至关重要.

研究的目的:

  • 开发一个具有成本效益的,小型的,可编程的系统,用于专门的材料变形测试.
  • 创建一个多功能设备,适应个别实验室要求和微观阶段.
  • 为了实现精确的单轴静态或循环负荷,用于材料分析.

主要方法:

  • 使用双极步进电机,螺丝和滑动线性阶段.
  • 使用Arduino微控制器进行编程和控制.
  • 组装了系统,专注于成本效益,不到100美元.
  • 通过显微镜验证了各种样品的性能和实时变形捕获.

主要成果:

  • 成功开发了一个功能性的微型可编程变形测试系统.
  • 证明了成本效益,组装成本低于100美元.
  • 在显微镜测试期间通过实时捕捉变形来验证设备性能.
关键词:
电纤维是电纤维的组成部分.图像成像是一种成像.机械性能 机械性能 机械性能微型拖拉机 微型拖拉机在PDMS中使用PDMS.压力应变是一种压力.

更多相关视频

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
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Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

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Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

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相关实验视频

Last Updated: Jan 20, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.9K
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
08:29

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Published on: September 9, 2020

3.5K
Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

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  • 确认了与微观阶段的实时成像的兼容性.
  • 结论:

    • 开发的系统为专门的材料变形测试提供了具有成本效益和微型解决方案.
    • 可编程性和微观兼容性满足了生物力学和材料科学领域的特定研究需求.
    • 这种硬件可以在微观阶段进行先进的材料分析,克服商业设备的局限性.