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相关概念视频

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics10:42

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

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Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical...
3.4K
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

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Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and...
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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

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We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based...
16.3K
A Paper-Based Immunoassay for the Detection of Immunoglobulin G04:48

A Paper-Based Immunoassay for the Detection of Immunoglobulin G

575
The video demonstrates a paper-based immunoassay, involving amine-functionalized cellulose discs cross-linked with glutaraldehyde. Covalent immobilization of IgG-Fc-specific capture antibodies, followed by the introduction of immunoglobulin G and horseradish peroxidase-conjugated detection antibodies, results in a color change confirming the presence of immunoglobulin...
575
Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen06:00

Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen

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Addressing urgent dengue diagnostic needs, here we introduce a smartphone app-integrated Dengue NS1 Paper-based Analytical Device (DEN-NS1-PAD) for quantifying Dengue NS1 antigen concentration in clinical serum/blood samples. This innovation enhances dengue management by aiding clinical decision-making in various healthcare settings, even resource-limited ones.
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Paper-based Devices for Isolation and Characterization of Extracellular Vesicles11:53

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

11.9K
This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited...
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相关实验视频

Updated: Jan 20, 2026

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
10:42

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

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基于纸张的诊断试验组件的开源CNC工作站.

Lucy Tecle1, Shannon Riegle2, Andrew Piepho1

  • 1Purdue University, 610 Purdue Mall, West Lafayette, IN 47907, United States.

HardwareX
|January 19, 2026
PubMed
概括

一个新的低成本,开源的工作站使用重新设计的CNC机器自动组装基于纸张的诊断. 这一创新提高了开发医疗点测试的准确性和可访问性.

科学领域:

  • 生物医学工程 生物医学工程
  • 诊断技术 诊断技术的使用
  • 开源硬件是开源的硬件.

背景情况:

  • 基于纸质的诊断为医疗点的测试提供了潜力,但手工组装引入了变化.
  • 当前的自动化解决方案往往昂贵且专有,限制了可访问性.

研究的目的:

  • 开发一个低成本的,开源的自动化工作站,用于组装基于纸张的诊断分析.
  • 为了提高纸质诊断的制造精度和可重复性.

主要方法:

  • 重新设计了一台三轴计算机数控 (CNC) 机器,配备了基于Arduino的控制器和GRBL固件.
  • 集成了一个定制的真空端效应器,用于处理微妙的测试部件.
  • 在各种基于纸张的诊断格式 (测量棒,LFA,双重免疫测试) 中验证了性能.

主要成果:

  • 实现了0.5-0.6mm的线性定位精度和可接受的角度偏差 (1-3°).
  • 与手工组装测试相比,证明了统计学上无法区分的对照线强度.
  • 将工作站成本降低到900美元以下,提高了可访问性.

结论:

  • 开源的CNC工作站能够以经济实惠的价格,自动组装基于纸张的诊断.
关键词:
自动化制造自动化制造自动化制造计算机数控 (CNC) 是指计算机的数控.低成本的仪器仪表使用.开源的硬件是开源的.基于纸质的诊断系统

更多相关视频

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

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相关实验视频

Last Updated: Jan 20, 2026

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
10:42

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

3.4K
Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

6.7K
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

16.3K
  • 这个平台为学术实验室,初创公司和去中心化环境提供了原型设计和扩展.
  • 保存的诊断性能验证了系统的早期研究和开发.