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Updated: Jan 20, 2026

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对匹配的冷和甲固定氨酸嵌入式结直肠癌组织进行比较空间全转录组分析

Kullanist Thanormjit^1,2, Thanawat Suwatthanarak^1,2, Tantip Arigul^3,4

¹Siriraj Cancer Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.

Biochemistry and biophysics reports

|January 19, 2026

概括

空间转录学揭示了结直肠癌 (CRC) 的异质性. 甲固定嵌 (FFPE) 样本提供了卓越的细胞细节,而新鲜冷 (FF) 和快速冷 (SF) 样本显示高基因表达,所有方法都识别了关键的CRC签名.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

空间转录学对于理解结直肠癌 (CRC) 中的瘤微环境异质性至关重要.
有限的数据存在,比较不同的CRC样品类型和保存方法 (FFPE,FF,SF).

研究的目的:

为了比较甲固化嵌 (FFPE),新鲜冷 (FF) 和快速冷 (SF) 大肠直肠癌 (CRC) 样本类型的优缺点.
评估基因表达特征和细胞形态分辨率,使用空间转录学在不同的保存方法.

主要方法:

在FFPE,FF和SF样本中的36个组织区域中分析基因表达特征,这些样本来自第四阶段的CRC患者.
详细的组织学评估和空间转录学分析来比较样本质量.
全转录组空间分析以确定基因表达模式和细胞异质性.

主要成果:

FFPE样本提供了细胞形态的增强分辨率和更详细的基因表达和瘤微环境中的细胞类型识别.
FF和SF组织表现出高水平的CRC基因表达.
在FFPE,FF和SF样本中观察到与关键CRC生物过程相关的顶级基因的显著重叠.

结论:

跨不同CRC组织保存方法的空间转录学分析揭示了内异质性.
每种保存方法 (FFPE,FF,SF) 都为结直肠癌的分析提供了特定的优势.
选择组织保存方法对于全面的CRC转录组分析至关重要.

关键词:

结肠直肠癌是一种癌症.固定在形式素中的嵌入式抛.新鲜的冷的快速冷的冷的快速冷.空间整体的转录学.

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