相关实验视频

Updated: Jan 20, 2026

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

韩国红人参对HIV-1env序列长度和CpG二核酸的作用

Jinny Lee¹, Seunghee Baek², Young-Keol Cho¹

¹Departments of Microbiology, Asan Medical Center, University of Ulsan College of Medicine, Republic of Korea.

Journal of ginseng research

|January 19, 2026

概括

在HIV-1患者的长期韩国红人参 (KRG) 治疗影响HIV-1 env基因. KRG与env序列长度和CpG700含量有关,特别是在长时间使用时.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

韩国红人参 (KRG) 显示对HIV-1患者的长期益处.
KRG对HIV-1病毒基因特征的影响尚不清楚.

研究的目的:

调查长期KRG治疗是否会影响HIV-1 env基因的序列长度 (SL) 和CpG700含量.
为了比较这些基因标记在KRG-naive和KRG经验的HIV-1患者之间.

主要方法:

分析了200名患者的880个HIV-1 env基因序列,平均时间为8.0年.
在KRG-naive和KRG-experienced组之间比较SL和CpG700内容.
使用嵌套PCR或RT-PCR进行序列获取.

主要成果:

在KRG经验丰富的患者中观察到SL和疾病持续时间之间的更强的相关性.
在多个样本的患者中,CpG700含量与疾病持续时间和KRG治疗有显著的相关性.
随着随访时间的延长,KRG和CpG700之间的关联得到了加强.

结论:

艾滋病毒-1 env 基因 SL 和 CpG700 含量与 KRG 治疗有显著的关联.
随着KRG的广泛使用,KRG和CpG700内容之间的联系变得更加明显.

关键词:

在CpG中包含的内容疾病持续时间.艾滋病毒-1 env 序列长度韩国红人参是韩国的红人参.

更多相关视频

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach

Prediction of HIV-1 Coreceptor Usage Tropism by Sequence Analysis using a Genotypic Approach

Published on: December 1, 2011

相关概念视频

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1...

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5...

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

The prediction of the coreceptor usage of HIV-1 is required for the administration of a new class of antiretroviral drugs, i.e. coreceptor antagonists. It can be performed by sequence analysis of the env gene and subsequent interpretation through an internet based interpretation system...

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env...

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Quantifying levels of HIV-1 RNA in plasma and sequencing single HIV-1 genomes from individuals with viral loads below the limit of detection (50-75 copies/ml) is difficult. Here we describe how to extract and quantify plasma viral RNA using a real time PCR assay that reliably measures HIV-1 RNA down to 0.3 copies/ml and how to amplify viral genomes by single genome sequencing, from samples with very low viral...

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein...