自然发生的反Jka:扩大证据超越敏感化
Ardenne Martin1, Katrina J Jiang1, Huseyin Kilic1
1Department of Pathology, University of South Alabama, Mobile, AL, USA.
概括
在没有事先敏感化的情况下,在两名患者身上发现了天然存在的抗Jka Kidd抗体. 固相红细胞粘附性测试检测到这些抗体,这些抗体被传统方法遗漏了,强调了其对输血安全的重要性.
科学领域:
- 输血医学 输血医学
- 免疫学 免疫学 免疫学
- 血液银行 血液银行
背景情况:
- 红细胞 (RBC) 抗体通常在输血或怀孕后形成.
- 自然存在的抗体产生时没有敏感化,通常针对 ABO 或易斯抗原.
- 基德系统抗体具有临床意义,通常是IgG,很少自然发生.
研究的目的:
- 调查自然存在的抗Jka抗体的发生和检测.
- 评估不同血清学方法在识别这些抗体方面的有效性.
主要方法:
- 使用自动化血凝和手动管道方法进行常规的输血前检测.
- 通过标准三细胞面板和固相红细胞粘附 (SPRCA) 进行抗体查.
- 使用PEG增强的间接抗球蛋白试验 (IAT) 进行表型和比较测试.
主要成果:
- 两名接受SPRCA检测的抗Jka药物的患者,尽管没有输血或怀孕史.
- 在SPRCA中观察到抗Jka的剂量效应,但使用PEG增强的IAT未能检测到.
- 自控和直接抗球蛋白试验呈阴性,没有发现其他显著抗体.
结论:
- 反Jka可以自然出现,独立于已知的敏感事件.
- SPRCA有效地检测弱或正在发展的抗体,这些抗体被传统的管道方法遗漏了.
- 对自然存在的基德抗体的认识对于输血中精确的血液选择至关重要.
相关概念视频
The Evidence for Evolution
47.6K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
47.6K
05:57Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment
23.8K
We demonstrate a method to collect magnetotactic bacteria (MTB) that can be applied to natural waters. MTB can be isolated and enriched from sediment samples using a relatively simple setup that takes advantage of the bacteria's natural magnetism. Isolated MTB can then be examined in detail using both light and electron...
23.8K
09:01An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen
12.6K
Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.
12.6K
06:53Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
3.6K
The present protocol shows how to differentiate CD3−/CD45+CD56+ cells with mild cytotoxicity from human expanded potential stem cells (hEPSCs) under both 3D and 2D culture conditions. This allows for routine phenotypical validation without the destruction of the complex microenvironment.
3.6K
Nature and Nurture
22.2K
Many human characteristics, like height, are shaped by both nature—in other words, by our genes—and by nurture, or our environment. For example, chronic stress during childhood inhibits the production of growth hormones and consequently reduces bone growth and height. Scientists estimate that 70-90% of variation in height is due to genetic differences among individuals, and 10-30% of variation in height is due to differences in the environments that individuals experience,...
22.2K
What is Natural Selection?
125.9K
Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
125.9K


