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相关概念视频

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the...
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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation12:26

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This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal...
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

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Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood...
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Identification of Circular RNAs using RNA Sequencing08:25

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Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

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This protocol describes the isolation and quantification of high-density lipoprotein small...
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相关实验视频

Updated: Jan 20, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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循环RNA:从非编码调节器到功能蛋白编码器.

Xinwei Zhang1,2,3, Hongyan Wu2,3,4,5, Xuechuan Hong2,3,4,5

  • 1School of Pharmacy, Shandong Second Medical University, Weifang, 261053, China.

Pharmaceutical science advances
|January 19, 2026
PubMed
概括

循环RNAs (circRNAs) 是独特的非编码RNA,具有多种功能. 这篇评论涵盖了它们的生物学,传递和在精密医学,基因治疗和疫苗中的应用.

关键词:
生物功能 生物功能这是循环RNARNA.交付策略的交付策略基因治疗是一种基因疗法.结构特征 结构特征 结构特征

更多相关视频

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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

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相关实验视频

Last Updated: Jan 20, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.2K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • 循环RNAs (circRNAs) 是一种非编码RNAs类,具有独特的共价封闭结构.
  • 与线性RNA相比,它们具有不同的生物特性和功能作用.
  • circRNAs正在成为生物研究中的重要分子.

研究的目的:

  • 总结一下最近在circRNA研究中的进展.
  • 强调circRNAs的基本机制和翻译潜力.
  • 为 circRNA 生物学和临床应用提供全面的视角.

主要方法:

  • 关于circRNA发现,合成和净化的文献综述.
  • 分析circRNA传递系统,生物功能和应用.
  • 讨论临床翻译的挑战和未来前景.

主要成果:

  • 详细概述circRNA特征和生物发生.
  • 探索各种生物功能和circRNAs的应用.
  • 确定临床翻译的关键领域,包括精准医学,基因疗法和个性化疫苗.

结论:

  • circRNAs代表了一个快速发展的领域,具有显著的翻译潜力.
  • 将分子机制与临床应用相结合,对于未来的进步至关重要.
  • circRNAs对开发新的治疗策略和个性化医疗方法充满希望.