RNA:

Weikai Yan1, Xiaocheng Weng1

  • 1College of Chemistry and Molecular Sciences, State Key Laboratory of Metabolism and Regulation in Complex Organisms, Department of Otorhinolaryngology-Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430072, China.

PubMed
概括

通过修改RNA分子,RNA基编辑可以精确纠正基因突变. 这篇评论详细介绍了RNA编辑系统,类型和遗传疾病的治疗应用.

相关概念视频

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution02:58

CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution

This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide...
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A Nonsequencing Approach for the Rapid Detection of RNA Editing08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.
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RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes09:19

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

A highly sensitive ribozyme-based assay, applicable to high-throughput screening of chemicals targeting the unique process of RNA editing in trypanosomatid pathogens, is described in this paper. Inhibitors can be used as tools for hypothesis-driven analysis of the RNA editing process and ultimately as...
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

A significant barrier to technologies like CRISPR is the off-target events that can disrupt vital genes. 'Circularization for In Vitro Reporting of Cleavage Effects by Sequencing' (CIRCLE-seq) is a technique designed to identify unintended cleavage sites. This method maps the genome-wide activity of CRISPR-Cas9 with high sensitivity and without...
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the...
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