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Updated: Jan 20, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

终端修复导致在无细胞DNA测序库中的甲基化低估

Tobin E Groth¹, Andrew A Mishin¹, Varsha Rao¹

¹Claret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA, 95066, USA.

The journal of liquid biopsy

|January 19, 2026

概括

无细胞DNA (cfDNA) 终端修复偏差甲基化推断. 具有末端修复的双链cfDNA库显示甲基化精度降低,与保持完整性的单链库不同.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

无细胞DNA (cfDNA) 甲基化测序对于确定原生组织和染色质结构至关重要.
图书馆为cfDNA分析准备工作流程通常涉及最终修复步骤.

研究的目的:

调查图书馆准备期间终端修复对cfDNA甲基化推断的影响.
为了比较使用单链与双链cfDNA库来确定起源组织的准确性.

主要方法:

匹配的单链和双链DNA库是从相同的cfDNA提取物中准备的.
在两种类型的图书馆中分析了推断的CpG甲基化水平.
评估了起源组织预测的准确性.

主要成果:

双链cfDNA库中的终端修复显著减少了全球推断的CpG甲基化.
这种甲基化推断的减少导致确定原产组织的准确性降低.
在双链图书馆中修剪读取终端部分纠正了偏差,但减少了覆盖范围,并丢失了碎片数据.
单链cfDNA库,其中省略了终端修复,保留了甲基化准确性和碎片信息.

结论:

终端修复是一个关键的步骤,它在cfDNA甲基化测序中引入偏差,影响起源组织的准确性.
单链图书馆制备是cfDNA甲基化分析的优越方法,保留了关键的表观遗传和碎片信息.

关键词:

没有细胞的DNA (cfDNA)双链DNA (dsDNA) 库的准备工作.终端维修是一项终端维修.碎片组学是一种碎片组学.甲基化序列的排列方式核位定位的核位定位单链DNA (ssDNA) 库的准备工作.原产地组织 (TOO)

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