相关实验视频

Updated: Jan 20, 2026

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

加斯德林介导的线粒体RNA释放在增强微生物感染期间的二次免疫反应中的作用

Uzair Afaq¹, Muhammad Suhaib Qudus¹, Siyu Liu¹

¹State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

Frontiers in immunology

|January 19, 2026

概括

细胞质RNA的释放会触发炎症. 加斯德明D (GSDMD) 和加斯德明E (GSDME) 孔隙形成释放线粒体RNA (mtRNA),激活病毒干扰素刺激基因激活器 (VISA) 途径,驱动炎症组织损伤.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

细胞质RNA作为损伤相关分子模式 (DAMP),但其释放机制和炎症中的作用尚不清楚.
了解DAMP释放对于炎症疾病研究至关重要.

研究的目的:

阐明细胞质RNA释放的机制及其在炎症反应中的作用.
调查加斯德明D (GSDMD) 和加斯德明E (GSDME) 在mtRNA释放和随后的炎症中的参与.

主要方法:

用脂聚糖 (LPS) 和尼格里 (Ng) 或膀性口炎病毒 (VSV) 刺激细胞.
气体皮质D (GSDMD) 和气体皮质E (GSDME) 的激活和孔隙形成的分析.
评估线粒体RNA (mtRNA) 释放及其被病毒干扰素刺激基因激活剂 (VISA) 识别.
使用了GSDMD/GSDME抑制和VISA淘汰 (VISA-/-) 鼠标模型.

主要成果:

LPS/Ng激活了GSDMD,而VSV则激活了GSDME. LPS/Ng激活了GSDMD,而VSV激活了GSDME.
无论是GSDMD还是GSDME都形成了线粒体毛孔,释放mtRNA到细胞质中.
释放的mtRNA激活了VISA传感器,诱导了二次炎症反应.
抑制GSDMD/GSDME或淘汰VISA可以减少炎症.

结论:

GSDMD和GSDME调解mtRNA释放,启动一个依赖VISA的炎症级联.
针对GSDMD,GSDME或VISA途径为涉及炎症的微生物传染病提供了潜在的治疗策略.

关键词:

在 GSDMD 分裂.在VISA途径中介的二次炎症反应中.免疫反应的免疫反应.线粒体功能障碍线粒体功能障碍释放 mtRNA 的时间.

更多相关视频

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Published on: November 27, 2014

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

相关概念视频

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

This protocol describes a high-throughput approach for evaluating plant ion leakage, peroxidase activity, and callose production in the same sample.

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in...

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine...

Humoral Immune Responses

Humoral Immune Responses

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...