相关实验视频

Updated: Jan 20, 2026

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility
06:11

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

2.0K

在长期的hiPSCs培养过程中,经过工程设计的LINC MIR503HG载荷的细胞外囊泡保持了干性和多能性

Jiaqi Zhu1,2, Xiaoren Zhu3,4, Wei Feng2,5

  • 1Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.

Bioactive materials
|January 19, 2026
PubMed
概括

研究人员开发了MIR503HG-EVs,以在长时间培养期间保持人类诱导多能干细胞 (hiPSC) 的干细胞. 这种新的方法保留了hiPSC的多能性和差异化潜力,这对于再生医学应用至关重要.

关键词:
不同化的差异化细胞外囊泡中的细胞外囊泡.人类诱导的多能干细胞干细胞.长长的非编码RNAs.干细胞的干细胞性

更多相关视频

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

2.5K
Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses03:21

Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses

515

相关概念视频

An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells06:11

An Automated Culture System for Maintaining and Differentiating Human-Induced Pluripotent Stem Cells

Here, we present a protocol for an automated cell culture system. This automated culture system reduces labor and benefits the users, including researchers unfamiliar with handling induced pluripotent stem (iPS) cells, from the maintenance of iPS cells to differentiation into various types of...
2.0K
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this...
2.5K
Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses03:21

Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses

This video demonstrates the process of inducing human pluripotent stem cells to differentiate into neurons by transfecting them with engineered lentiviruses carrying a neurogenic transcription factor gene under the control of  tetracycline-responsive element, followed by treatment with a tetracycline derivative and maturation...
515
Generating and Maintaining Astrospheres Using Human Pluripotent Stem Cells03:07

Generating and Maintaining Astrospheres Using Human Pluripotent Stem Cells

The video demonstrates a method to generate astrospheres from progenitors from human pluripotent stem cells. It involves the growth and proliferation of stem cells with nutrient media containing growth factors and kinase inhibitors. These cells then aggregate and differentiate into astrocytes, forming...
413
Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells04:54

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells

This protocol describes all procedures, from culturing human adipose-derived mesenchymal stem cells (ADSCs) and collecting supernatant to extracting extracellular vesicles (EVs) using...
1.2K
A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells09:10

A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

This protocol demonstrates how to isolate human umbilical cord-derived mesenchymal stem cells' small extracellular vesicles (hUC-MSC-sEVs) with a simple lab-scale benchtop setting. The size distribution, protein concentration, sEVs markers, and morphology of isolated hUC-MSC-sEVs are characterized by nanoparticle tracking analysis, BCA protein assay, western blot, and transmission electron microscope,...
1.9K