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Protein Engineering by Yeast Surface Display
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通过代谢工程,通过油性红酵母Rhodotorula toruloides生物合成帕丘
Xiaochun Zheng1, Yajun Li1, Zhenhua Liu2
1College of Enology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Synthetic and systems biotechnology
|January 19, 2026
概括
使用工程 *Rhodotorula toruloides* 的微生物生产提供了一种可持续的替代植物提取方法. 这项研究通过代谢工程和优化培养提高了帕丘的产量,在生物反应器中实现了显著的生产水平.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 帕丘,一种有价值的甲,由于植物资源有限和季节性变化,面临生产挑战.
- 微生物发酵是一种可持续和环保的替代品,用于生产类.
研究的目的:
- 为了对油性酵母*Rhodotorula toruloides*进行工程设计,以有效地产生微生物的patchoulol.
- 优化新陈代谢途径和种植条件,以最大限度地提高patchoulol产量.
主要方法:
- 使用仿真酶和原生FPPS构建了patchoulol生物合成途径.
- 通过梅瓦酸盐 (MVA) 路径重新配置和增强NADPH回收来简化中间供应.
- 优化了烯生物合成,培养条件,并进行了生物反应器发酵.
主要成果:
- 在摇瓶条件下,实现了724.8 mg/L的增强型帕丘醇产量.
- 在3L生物反应器中达到1.31g/L的最终patchoulol度,产量为13.8 mg/g的葡萄糖.
- 通过代谢工程,在生产率和产量方面取得了显著的改善.
结论:
- 确立了*Rhodotorula toruloides*作为可持续的patchoulol微生物合成的可行的宿主.
- 这项研究为微生物生产其他有价值的甲提供了基础.
- 工程酵母菌株和优化的发酵过程为天然产品制造提供了可扩展和环保的方法.
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