相关实验视频

Updated: Jan 20, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

基于自制净化柱的LC-MS/MS方法的开发和验证,用于同时确定基于大米的产品中的新型有机酸盐

Haiyan Zhou^1,2, Jie Cao¹, Fang Xiao¹

¹School of Food and Bioengineering, Food Microbiology Key Laboratory of Sichuan Province, Xihua University, Chengdu, Sichuan 610039, China.

Food chemistry: X

|January 19, 2026

概括

有机酸 (OPE) 是在米产品中发现的污染物. 一种新的LC-MS/MS方法简化了检测,揭示了广泛的OPE污染,特别是在米中.

科学领域:

环境化学环境化学
分析化学分析化学

背景情况:

有机酸 (OPE) 是普遍存在的阻燃剂和增塑剂.
新兴有机污染物,OPE显示有关食品,特别是米制品中的污染水平.

研究的目的:

开发一种简化且具有成本效益的方法,用于在米制品中同时确定五种OPE.
评估基于大米的食品中OPE的污染水平和分布.

主要方法:

开发一种液体染色学-双重质谱法 (LC-MS/MS) 方法.
使用各种填充剂优化净化,以简化样品的预处理.
验证该方法的线性,检测极限,量化极限,恢复和矩阵效应.

主要成果:

LC-MS/MS方法显示出优异的线性 (R2>0.99) 和改进的检测/量化极限.
实现了高恢复率 (62.16-104.83%) 和可以忽略不计的矩阵效应 (<20%).
在大米样本中广泛检测到OPE,与抛光大米相比,大米中的度明显更高.

结论:

开发的LC-MS/MS方法适用于同时确定基于大米的产品中的OPE.
基于大米的食品,特别是大米,是OPE污染的重要储存库.
三乙烯酸盐 (TEP) 在中国南部的样中被确定为主要的OPE污染物.

关键词:

污染监测是指对污染的监测.这是LC-MS/MS.有机酸盐 Ester 是一种有机酸盐.大米大米大米大米大米大米是指大米中的一种.

更多相关视频

Size Exclusion and Affinity Chromatography

Size Exclusion and Affinity Chromatography

Published on: April 30, 2023

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

相关概念视频

Chromatography-based Biomolecule Purification Methods

Chromatography-based Biomolecule Purification Methods

In biochemistry, chromatography-based purification methods are employed to isolate compounds from a complex mixture. Two such methods used commonly by biochemists are size-exclusion chromatography and affinity chromatography. In size-exclusion chromatography, a column packed with porous beads separates components of a mixture based on size. On the other hand, affinity chromatography allows for a more specific separation of biomolecules by using a column that is composed of stationary phase,...

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Here, we present a protocol for an efficient single step purification of the active untagged human ten-eleven translocation-2 (TET2) 5-methylcytosine dioxygenase using ion-exchange chromatography and its assay using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based...

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in...

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Here, we describe an efficient and reproducible strategy to produce, titer, and quality-control batches of adeno-associated virus vectors. It allows the user to obtain a vector preparation with high-titer (≥1 x 1013 vector genomes/mL) and a high purity, ready for in vitro or in vivo...

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Here, we describe a method for the real-time detection of apoplastic reactive oxygen species (ROS) production in rice tissues in pathogen-associated molecular pattern-triggered immune response. This method is simple, standardized, and generates highly reproducible results under controlled...

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical...