在短期抗生素暴露后感染性内心炎的长期培养消极性:一个案例报告
Natsuki Mohri1, Kiyofumi Ohkusu2, Nobuyuki Yoshitani3
1Department of Infectious Diseases, Hyogo Prefectural Harima-Himeji General Medical Center, Himeji, JPN.
Cureus
|January 19, 2026
概括
培养负性传染性内心炎 (IE) 具有挑战性,即使在服用抗生素后也是如此. 像16S rRNA PCR这样的分子工具可以识别细菌,在血液培养结果阴性时指导IE的治疗.
科学领域:
- 传染性疾病 传染性疾病
- 微生物学 微生物学
- 心脏病学 心脏病学
背景情况:
- 血培养阴性传染性内心炎 (IE) 存在重大诊断挑战.
- 以前的抗生素暴露是IE中阴性血液培养的常见原因.
- 延迟IE诊断可能会导致严重的并发症.
研究的目的:
- 报告一个具有显著先前抗生素暴露的患者的培养负IE病例.
- 突出分子诊断工具在培养负IE中识别病原体的实用性.
- 强调即使经过长时间的无抗生素期,考虑IE的重要性.
主要方法:
- 一个63岁妇女的病例报告,怀疑患有IE.
- 审查之前的抗生素治疗 (莱沃克萨辛,塞夫特里亚,塞法索林).
- 血液和膜培养物的分析,以及16S rRNA聚合酶链反应 (PCR).
主要成果:
- 血液和门培养对细菌生长呈负面影响.
- 16S rRNA PCR成功识别了 *Streptococcus mitis * (组). 这是一个很好的方法.
- 针对性抗生素治疗是基于PCR结果开始的.
结论:
- 超过10天的抗生素暴露仍然会导致阴性血液培养.
- 16S rRNA PCR 是诊断培养负IE的一个有价值的工具.
- 在具有挑战性的病例中,临床医生必须保持对IE的高度怀疑指数,而不管之前是否使用过抗生素.
相关概念视频
In Vitro Calcium Imaging in Short-Term and Long-Term Rat Hippocampal Cultures
579
Source: Calvo-Rodríguez, et al. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons. J. Vis. Exp. (2015). This video demonstrates the use of fluorescence imaging to measure cytosolic calcium levels in short-term and long-term rat hippocampal cultures. By comparing young and aged neurons, it highlights how increased L-type voltage-gated calcium channel density and prolonged activation in aged neurons result in calcium overloading, a...
579
06:41Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
2.5K
We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter...
2.5K
Endocarditis II: Clinical Features of Infective Endocarditis
434
Endocarditis can present various clinical features depending on the causative organism and the patient's underlying health conditions. Initially, the clinical features of infective endocarditis develop gradually, presenting with nonspecific symptoms that can be easily mistaken for other illnesses.General SymptomsEarly symptoms of infective endocarditis are fever, chills, weakness, malaise, fatigue, and weight loss. These symptoms reflect the systemic nature of the infection and the body's...
434
02:09Synaptic Modulation in Drosophila After Exposure to Prolonged Light
507
This video demonstrates the rearing of Drosophila melanogaster under specific light conditions to study the role of Bruchpilot proteins in neurotransmitter release and the resulting modulation of synaptic...
507
09:14Short-Term Free-Floating Slice Cultures from the Adult Human Brain
9.6K
A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain...
9.6K
Inducing Hearing Loss in Mice Through Prolonged High-Volume Sound Exposure
552
Source: Lee, D. et. al., Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage. J. Vis. Exp. (2023)This video demonstrates the procedure of noise-induced hearing loss in mice. High-volume sound damages hair cells in the inner ear and disrupts auditory pathways, resulting in hearing...
552

