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Updated: Jan 20, 2026

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编码relaxin-2的循环RNA作为肝纤维化潜在治疗方法

Jiewen Zhong^1,2, Zheyu Zhang^1,2, Lixing Xiao²

¹Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Molecular therapy. Nucleic acids

|January 19, 2026

概括

循环RNAs (circRNAs) 提供了一个稳定的,低免疫性药物平台. 一种编码人类放松素-2 (cRLN2) 的新型circRNA有效地治疗了小鼠肝纤维化,并具有出色的安全性.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
药物开发药物开发

背景情况:

循环RNAs (circRNAs) 正在作为一种新的治疗方式获得关注.
与线性RNA相比,它们提供了诸如增强的稳定性和降低的免疫性等优势.
人类放松素-2 (RLN2) 是一种类激素,在纤维性疾病中具有治疗潜力.

研究的目的:

开发和评估一种编码人类放松素-2 (cRLN2) 的循环RNA,用于肝纤维化治疗.
为了比较cRLN2的疗效和表达特征与修改的线性mRNA和放松蛋白.
评估cRLN2纳米粒子输送的体内安全性和耐受性.

主要方法:

开发一种编码人类放松素-2 (cRLN2) 的circRNA结构.
在体外比较cRLN2和修饰线性mRNA用于放松素表达.
在健康和纤维化的小鼠模型中,通过纳米粒子介导的cRLN2的输送.
对肝星细胞激活,原沉积和毒性的评估.

主要成果:

与线性mRNA相比,cRLN2在体外表现出更优越,更持续的放松素表达.
纳米粒子输送cRLN2导致长时间的体内放松素转化,免疫性较低.
cRLN2治疗显著降低了肝纤维化标志物,包括肝星细胞激活和原沉积.
相比之下,直接的放松蛋白治疗显示出有限的抗纤维化作用.
毒性评估证实了cRLN2在小鼠中的安全性和耐受性.

结论:

cRLN2是一种有效的平台,可在体内持续治疗蛋白质表达.
cRLN2显示出治疗肝纤维化的显著潜力,具有有利的安全性.
循环RNAs代表了纤维性疾病的有前途的新疗法策略.

关键词:

MT:临床应用临床应用圆形的RNARNA是一个圆形的RNA.肝脏纤维化肝脏纤维化它们是mRNARNA.放松的放松的放松翻译翻译翻译翻译翻译翻译

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