相关实验视频

Updated: Jan 20, 2026

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

重组CALR极化并激活瘤中的巨细胞

Simin Manole¹, Neil S Forbes^2,3,4

¹Molecular and Cellular Biology Program, University of Massachusetts Amherst, Amherst, MA, USA.

Molecular therapy. Oncology

|January 19, 2026

概括

重组甲基素 (CALR) 作为免疫辅助剂,通过促进免疫细胞透和激活,将冷瘤转化为热瘤. 这种方法增强了抗原呈现,并提高了癌症免疫疗法的疗效.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

免疫疗法对于免疫性冷瘤通常是无效的.
使用损伤相关分子模式 (DAMPs) 模拟免疫细胞死亡 (ICD) 可以增强免疫细胞活性.
卡尔雷丘林 (CALR) 是一种已知的DAMP,具有潜在的辅助性.

研究的目的:

研究重组CALR作为免疫辅助剂的潜力.
为了确定CALR的管理是否可以触发白细胞透和激活瘤透巨细胞.
在体内评估CALR对瘤生长和免疫细胞群的影响.

主要方法:

经过工程Salmonella来产生重组CALR.
培养了巨细胞和树突细胞,以评估CALR对免疫细胞表型和激活标记物的影响.
给瘤小鼠注射复合CALR,以评估其体内疗效和对瘤微环境的影响.

主要成果:

再组合CALR在体外将巨细胞转移到炎症M1表型.
由CALR刺激的巨细胞激活了树突细胞,并诱导了B7共刺激标记物的表达.
在小鼠中,CALR降低了瘤体积,增加了白细胞和炎症性巨细胞的透,并增强了T细胞的激活和透.

结论:

再组合CALR通过诱导人工ICD作为有效的免疫辅助剂起作用.
CALR促进了巨细胞的两极分化和T细胞的激活,这对于抗瘤免疫是至关重要的.
这一策略有望改善抗原呈现并放大癌症免疫疗法的疗效.

关键词:

MT: 定期发行定期发行与损伤相关的分子模式.免疫性细胞死亡免疫性细胞死亡炎症反应是一种炎症反应.巨细胞是什么?巨细胞是什么?重组蛋白质疗法是一种重组蛋白质疗法.

更多相关视频

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Published on: January 7, 2019

相关概念视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling...

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Here, we present a method using permeable membrane supports to facilitate the study of non-contact paracrine signaling used by tumor cells to suppress the immune response. The system is amenable to studying the role of tumor-secreted factors in dampening macrophage...

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage...

Differentiation and Polarization of Monocyte-Derived Cells into Macrophage-Like Cells

Differentiation and Polarization of Monocyte-Derived Cells into Macrophage-Like Cells

In this video, we demonstrate a procedure to induce the differentiation and polarization of peripheral blood-derived monocytes into M1 or M2 macrophages. Incubation with granulocyte-macrophage colony-stimulating factor, lipopolysaccharide, and interferon‐gamma yields pro-inflammatory M1 macrophages, while incubation with macrophage colony-stimulating factor and interleukin-4 induces anti-inflammatory M2 macrophage...

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional...

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

M2-like tumor-associated macrophages (TAM) are associated with tumor progression and poor prognosis in cancer. This protocol serves as a detailed guide to reproducibly differentiate and polarize THP-1 monocyte-like cells into M2-like macrophages within 14 days. This model is the basis to investigate the anti-inflammatory effects of TAM within the tumor...