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通过代谢工程进行植物天然产品生物合成

Caibin Zhang^1,2, Jingcheng Shi^1,3, Rui Deng^1,2

¹State Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing, 100101, China.

Journal of integrative plant biology

|January 19, 2026

概括

植物代谢子是多酶复合体,控制代谢途径. 新的工程策略允许精确控制这些复杂物,以改善天然产品的合成和更安全的中间处理.

科学领域:

植物生物化学和分子生物学.
代谢工程和合成生物学.

背景情况:

代谢子或多酶复合物越来越多地被认为是它们在调节植物代谢途径中的作用.
这些复合物促进基质通道,保护反应性中间体,并使有效的代谢流量控制成为可能.

研究的目的:

定义识别真实代谢子的标准,并综合证据证明它们在各种植物途径中的作用.
为研究体内代谢素的组成,动态和功能提供一个集成的工作流.
概述用于生物技术应用的可编程代谢子的工程策略.

主要方法:

亲和净化质谱 (AP-MS) /共免疫沉 (Co-IP) 和近距离标记以确定复杂的组成.
双分子光补充 (BiFC) /福斯特共振能量转移 (FRET) /分裂化酶试验用于研究蛋白质-蛋白质相互作用和动态.
以同位素稀释的代谢学来分析体内基质的道化.
用人工智能辅助的生成模型来设计酶结合剂和接口.

主要成果:

合成了有关代谢素参与蓝色基因糖化物,烯胺/黄胺,类化合物,类化合物,聚胺,杂草蛋白和辅酶通路的证据.
一个综合的工作流,结合多种技术被介绍为全面的代谢素分析.
工程代谢子的设计规则,包括膜定和隔间准,被提炼出来.
人工智能被提议用于优化代谢细胞内的酶排列.

结论:

代谢是一种强大的,可部署的技术,用于植物可编程的代谢流.
工程代谢器可以带来更安全的反应性中间体处理和增加有价值的自然产品的生产.
这项工作为推进植物生物技术中的代谢素研究和应用提供了一个框架.

关键词:

代谢工程是代谢工程.植物天然产品植物天然产品基质的道化道化合成生物学合成生物学

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