相关实验视频

Updated: Jan 20, 2026

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

8.2K

角膜巨细胞和肢体干细胞:眼睛表面再生中的新兴作用

Yi Mao1,2, Shangkun Ou2,3, Wei Si1

  • 1Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Annals of medicine
|January 19, 2026
PubMed
概括

巨细胞调节肢体干细胞,影响角膜健康和再生. 向巨细胞干细胞相互作用为治疗干细胞缺乏和恢复视力提供了一个有希望的策略.

关键词:
角质细胞巨细胞.免疫干细胞的相互作用这是一种炎症炎症炎症炎症.肢体干细胞 肢体干细胞巨细胞的两极分化眼睛表面的再生再生.

更多相关视频

Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration07:39

Author Spotlight: Advancing Research in Corneal Opacity Treatment and Regeneration

Published on: August 4, 2023

2.7K
A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.7K

相关概念视频

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy...
8.2K
Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model07:39

Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model

This protocol describes a method to induce an accurate and reproducible corneal and limbal alkali injury in a mouse model. The protocol is advantageous as it allows for an evenly distributed injury to the highly curved mouse cornea and...
2.7K
A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

The following article provides an easy, reproducible technique to effectively create a sustainable mouse model of limbal stem cell deficiency (LSCD). This animal model is useful in testing and comparing the efficacy of treatments for limbal stem cell...
8.7K
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

We report a technique for the fabrication of micropockets within electrospun membranes in which to study cell behavior. Specifically, we describe a combination of microstereolithography and electrospinning for the production of PLGA (Poly(lactide-co-glycolide)) corneal biomaterial devices equipped with...
12.9K
Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

This protocol describes a method to inflict an abrasion to the ocular surface of the mouse, and to follow the wound healing process thereafter. The protocol takes advantage of an ocular burr to partially remove the surface epithelium of the eye in anaesthetized...
21.3K
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice07:48

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of...
18.0K