银纳米粒子和温度对Palaemon pandaliformis的联合作用:一种基于毒性和生理生物标志物的方法

Presented here is a protocol for using silver nanoparticles to effectively ameliorate the acute symptoms of type II collagenase-induced osteoarthritis mice, including synovial inflammation, synovial hyperplasia, vascular hyperplasia,...

Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic...

Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal...

A facile protocol is presented to functionalize the surfaces of nanodiamonds with...

Liver macrophages, named Kupffer cells, are responsible for the capture of circulating nanoparticles. We describe here a method, of high cell purity and yield, for Kupffer cell isolation. The modified LDH assay is used here to measure the toxicity induced by carbon nanotubes in Kupffer cells.

Begin with a slurry containing bacterial cells treated with silver nanoparticles suspended in an exopolysaccharide matrix.Add a phosphate buffer to the slurry to maintain a stable pH.Next, introduce a solution containing glucose and a soluble redox indicator, then incubate the mixture. The redox indicator acts as an electron acceptor.During incubation, glucose and the redox indicator enter metabolically active bacterial cells.The cells utilize glucose during respiration, generating...