在基于生物传感器的未经处理的血分析中解决矩阵效应
Giusy Finocchiaro1, Markéta Bocková1, Jiří Homola2
1Institute of Photonics and Electronics of the Czech Academy of Sciences, Chaberská 1014/57, 182 00, Prague, Czech Republic.
Analytical and bioanalytical chemistry
|January 19, 2026
概括
这项研究解决了人类血中的矩阵效应,以使用表面等离子体共振生物传感器准确检测生物标志物. 一种新的策略改善了癌症生物标志物的检测极限,例如癌胚抗原.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 人类血对于诊断至关重要,但其复杂的矩阵会引起干扰.
- 矩阵效应,包括非特异性吸附和内源性抗体相互作用,阻碍了准确的低度生物标志物检测.
研究的目的:
- 用表面等离子体共振 (SPR) 生物传感器研究和区分人类血中的矩阵效应.
- 开发和应用一种策略,以减轻敏感生物标志物检测的矩阵效应.
主要方法:
- 使用了表面等离子体共振 (SPR) 生物传感器技术.
- 实施了一种结合连续注射,单面引用和抗免疫球蛋白抗体的策略.
- 应用了在未稀释的血中检测癌胚抗原 (CEA) 的策略.
主要成果:
- 成功区分了矩阵效应,包括非特异性吸附和抗体相互作用.
- 实现了CEA的低检测极限:12 ng/mL (直接检测) 和225 pg/mL (用金纳米颗粒进行三明治检测).
结论:
- 开发的策略有效地克服了基于SPR的诊断在人血中的矩阵效应.
- 能够在复杂的生物样本中灵敏而准确地检测癌症生物标志物,如CEA.
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