相关实验视频

Updated: Jan 20, 2026

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Published on: June 17, 2025

在异常性肺纤维化中,单细胞介导机制:早期干预的机会

Zhiguo Mao^1,2, Ying Liu^2,3, Chengfu Han^1,2

¹Department of Pharmacology, Henan University of Chinese Medicine, No. 156 Jinshui East Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.

Apoptosis : an international journal on programmed cell death

|January 19, 2026

概括

异形性肺纤维化 (IPF) 涉及到细胞外基质沉积. 单细胞数量升高表明预后不佳,这表明单细胞是IPF进展的关键驱动因素和新疗法的点.

科学领域:

肺部病理学肺部病理学
免疫学免疫学免疫学
病理学病理学病理学

背景情况:

异形性肺纤维化 (IPF) 是一种慢性肺病,其特征是不可逆转的细胞外基质沉积和结构破坏.
目前的治疗方法,如Pirfenidone和Nintedanib,只能减缓IPF的进展.
周围血液单细胞计数是IPF预后和死亡率的重要生物标志物.

研究的目的:

审查IPF中单细胞向肺部招募的机制.
为了研究单细胞在IPF病变发生中的作用.
为开发针对IPF的新型单细胞向疗法提供基础.

主要方法:

文献综述侧重于IPF中的单细胞招募途径.
在IPF中分化单细胞衍生物细胞 (巨细胞,树突细胞,纤维细胞) 的作用的分析.
综合当前关于单细胞参与IPF病理学的知识.

主要成果:

肺组织中单细胞的积累是IPF的一个持久特征,即使在早期阶段也很明显.
单细胞在肺内分化为各种细胞类型,巨细胞在疾病进展中发挥着关键作用.
化学吸引剂信号驱动单细胞向肺组织招募.

结论:

单细胞是IPF病变发生的核心参与者.
了解单细胞的招募和功能为早期IPF检测和干预提供了潜力.
向单细胞为开发IPF新型治疗策略提供了一个有希望的途径.

关键词:

化学因子 (chemokines) 是一种化学物质.细胞外矩阵是细胞外矩阵.异形性肺纤维化症异形性肺纤维化症巨细胞是一个巨细胞.单细胞 (Monocytes) 是一种单细胞.

更多相关视频

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Published on: May 5, 2023

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

相关概念视频

Refined Murine Model of Idiopathic Pulmonary Fibrosis

Refined Murine Model of Idiopathic Pulmonary Fibrosis

This protocol delineates a method for inducing pulmonary fibrosis in mice through repeated oropharyngeal administration of bleomycin, yielding pathological features closely resembling those of idiopathic pulmonary...

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

This protocol describes the isolation of pulmonary interstitial macrophages (IMs) and their adoptive transfer after IL-33 stimulation of the lung alveoli in a mouse model, which can facilitate the in vivo study of idiopathic pulmonary fibrosis...

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

This protocol demonstrates the oropharyngeal aspiration technique for use in the bleomycin murine model of pulmonary...

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis

Here, the method of inducing pulmonary arterial hypertension associated with pulmonary fibrosis (PF-PH) rat model by injecting bleomycin into the airway is introduced. We also provide a step-by-step approach to validate this animal...

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Various animal models of pulmonary fibrosis have been established using bleomycin to clarify the pathogenesis of pulmonary fibrosis and find new drug targets. However, most pulmonary fibrosis models targeting lung tissue have uneven drug administration. Here, we propose a model of uniform pulmonary fibrosis induced by nasal bleomycin...

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models

Here, we present a protocol to utilize micro-computed tomography (micro-CT) to quantify ventilated regions in a unilateral bleomycin-induced pulmonary fibrosis model via intratracheal instillation on the lesional...