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Updated: Jan 21, 2026

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
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Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

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改进CAR T细胞中的CRISPR-Cas9屏幕:用于图书馆准备的精细方法

Maider Garnica1, Patxi San Martin-Uriz2, Paula Rodriguez-Marquez2

  • 1Hemato-Oncology Program, Cima Universidad de Navarra, IdiSNA.

Journal of visualized experiments : JoVE
|January 19, 2026
PubMed
概括

这项研究优化了对仿真抗原受体 (CAR) T 细胞的集群定期间隔的短时间palindromic重复 (CRISPR) -Cas9查. 改进的协议增强了sgRNA检索,有助于发现基因点,以提高CAR T细胞治疗的疗效.

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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