红色复杂细菌DNA和牙周状况在可拆卸部分牙套佩戴器中的实时PCR量化
Enis Veseli1, Kelvin I Afrashtehfar2, Argjira Veseli3
1Department of Prosthodontics, Dental School, Faculty of Medicine, University of Prishtina; enis.veseli@uni-pr.edu.
Journal of visualized experiments : JoVE
|January 19, 2026
概括
可拆卸部分假牙 (RPD) 在支柱牙上显著增加红色复杂细菌 (RCB). 这种微生物转移可能会在牙周问题之前发生,突出显示需要监测RPD用户的口腔健康.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙周病学 牙周病学
- 牙修复 牙修复 牙修复 牙修复 牙修复
背景情况:
- 可拆卸部分假牙 (RPD) 改变了口腔微生物群.
- 红色复杂细菌 (RCB) 与牙周炎和全身性疾病有关.
- 在RPD患者中对RCB的研究有限.
研究的目的:
- 在RPD使用者的牙中量化RCB (Porphyromonas gingivalis,Tannerella forsythia,Treponema denticola).
- 评估支柱牙和非支柱牙的牙周状况.
- 将微生物负载与临床牙周指数相关联.
主要方法:
- 实时聚合酶连锁反应 (RT-PCR) 用于RCB量化.
- 从基线 (T0) 和插入后3个月 (T3) 的支柱牙样本中分离的DNA.
- 记录的临床参数:斑块指数 (PI),牙指数 (GI),探测深度 (PD),牙移动性 (TM).
主要成果:
- 从T0到T3对支柱牙的RCB负荷 (P. gingivalis,T. forsythia,T. denticola) 显著增加.
- 牙周参数显示,治疗后牙支柱与非支柱牙的升高不显著.
- 丹尼雷拉·福西提亚 (Tannerella forsythia) 显示出与牙周指数最强的正相关性.
结论:
- 短期使用RPD会导致RCB在支柱牙上的殖民性增加.
- 早期的微生物和炎症转移发生在RPD插入时.
- 建议进行进一步的监测,以防止临床上明显的牙周病变.
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