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Updated: Jan 21, 2026

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Ex Situ Normothermic Machine Perfusion of Donor Livers
Published on: May 26, 2015
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将连续脏置换疗法集成到现场正常热肝脏机器 perfusion 中
Jordi Vengohechea1, Soraya Rodríguez2, Alicia Izquierdo2
1Department of Surgery and Surgical Specializations, University of Barcelona; General & Digestive Surgery Service, Hospital Universitario La Paz; Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd).
Journal of visualized experiments : JoVE
|January 19, 2026
概括
结合连续置换疗法 (CRRT) 与肝移植的 ex situ normothermic 机器输液 (NMP),可以改善废物清除. 这种方法支持延长输液,并提高了移植移植的移植活力.
科学领域:
- 器官移植 器官移植 器官移植
- 机器 perfusion 技术 机器 perfusion 技术 机器 perfusion 技术 机器 perfusion 技术 机器 perfusion 技术 机器 perfusion 技术 机器 perfusion 技术
- 脏替代疗法是指脏替代疗法.
背景情况:
- 现场常热机器输液 (NMP) 支持肝移植的活力,但受到代谢废物积累的限制.
- 连续置换疗法 (CRRT) 可以管理溶液清除和恒温.
研究的目的:
- 描述一种将连续毒性血液过 (CVVHDF) 整合到ex situ肝脏NMP的方法.
- 评估一个独立的过电路对植入物稳定性和功能的影响.
主要方法:
- 描述了连接CVVHDF电路独立于肝脏NMP主电路的协议.
- 详细的物理连接,初始化,监控和霍夫曼用于压力模拟的使用.
- 我们比较了一种离线CVVHDF方法与直接在线集成.
主要成果:
- 独立的CVVHDF电路配置保持了血液动力学稳定性,并防止了过度的血管内压力.
- 与直接在线整合相比,外循环方法与减少移植有关.
- 保持了稳定的电路压力和有效的溶液清除率.
结论:
- 独立将CVVHDF集成到ex situ肝脏NMP中是一种安全和可重复的方法.
- 这种方法增强了溶液处理,并防止在延长 perfusion 期间移植胀.
- 该方法可适应其他 perfusion 系统,以改善移植物的保存.
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