来自Oreochromis niloticus (林奈斯,1758) 的新肌肉细胞系的开发和蛋白质体的表征
Vijay Lakshmi Sahoo1, A Sathiyanarayanan1, Nevil Pinto1
1Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Panch Marg, Off Yari Road, Versova, Andheri West, Mumbai, 400061, Maharashtra, India.
Differentiation; research in biological diversity
|January 19, 2026
概括
为水产养殖研究建立了一种新的Oreochromis niloticus肌肉 (ONM) 细胞系. 这种鱼细胞系支持实验室培养肉类和细胞水产养殖进步的体外研究.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 细胞生物学 细胞生物学
- 鱼类细胞系发展 鱼类细胞系发展
背景情况:
- 基因改进的养殖鱼 (GIFT) 对水产养殖至关重要.
- 开发稳定的鱼类细胞系对于推进细胞农业和体外研究至关重要.
研究的目的:
- 从Oreochromis niloticus (tilapia) 开发和表征一种新的肌肉细胞系.
- 评估细胞系是否适用于体外应用,包括实验室培养的肉类和细胞水产养殖.
主要方法:
- 用于细胞系建立的爆炸技术.
- 细胞培养优化涉及28°C的L-15介质,FBS和bFGF.
- 线粒体基因测序用于认证.
- 转化效率和蛋白质与蛋白质相互作用分析.
主要成果:
- 已建立的ONM细胞系保持在L-15介质中,5%的FBS和10 ng/ml的bFGF.
- 证明了19%的涂层效率和36小时的翻倍时间.
- 通过绿色光蛋白实现了6%的转化效率.
- 确定了关键通路 (胰岛素,WNT,VEGFA-VEGFR2),表明细胞分化.
结论:
- ONM细胞系是鱼肌肉研究的可行模型.
- 细胞系可以适应降低的血清条件,促进经济有效的研究.
- 这项开发为实验室培养的肉类和推进细胞水产养殖提供了一个平台.
更多相关视频
相关概念视频
Proteomics
9.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.4K
The Role of Actin and Myosin in Non-muscle Cells
4.7K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They are held...
4.7K
Classification of Skeletal Muscle Fibers
59.4K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
59.4K
Zygotic Development And Stem Cell Formation
6.6K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.6K
Sustainable Development
14.8K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
14.8K
Muscles of the Eye
4.0K
The muscles of the eye are sophisticated structures that control eye movement and focus, allowing for the precise and rapid adjustments necessary for vision. The human eye is controlled by ten muscles — six extraocular muscles, three intraocular muscles, and one primary eyelid retractor muscle.
Extraocular Muscles
The six extraocular muscles surround the eyeball and control its movements. They are responsible for a wide range of eye motions, including looking up, down, left, right, and...
Extraocular Muscles
The six extraocular muscles surround the eyeball and control its movements. They are responsible for a wide range of eye motions, including looking up, down, left, right, and...
4.0K


