样品储存时间和温度对马匹全血细胞计数的影响
M M Friend1, E M McGaffigan1, S M Hall1
1Department of Animal Science, College of Agricultural Sciences, Pennsylvania State University, 439 Shortlidge Road, University Park, PA 16802.
Journal of equine veterinary science
|January 19, 2026
概括
马类血液样本的稳定性受到储存温度和时间的影响. 建议在冷藏室 (4°C或8°C) 储存长达48小时,以进行准确的全血细胞计 (CBC) 分析.
科学领域:
- 兽医血液学 兽医血液学
- 马匹临床病理学
背景情况:
- 马类血液样本经常在偏远地区收集.
- 由于存储要求,延迟分析是常见的.
研究的目的:
- 评估不同储存温度和时间对马匹全血细胞计 (CBC) 参数的影响.
- 为了确定最佳的储存条件,以保持样本的完整性.
主要方法:
- 从10只四分之一马马的血液样本被存储在2°C,4°C,8°C和22°C.
- 使用IDEXX Procyte Dx在多个时间点进行了全血细胞计数 (CBC),时间长达72小时.
- 统计分析包括Kruskal-Wallis和Dunn的后期测试与本雅米尼-霍赫伯格校正.
主要成果:
- 细胞降解 (单细胞减少,基细胞增加) 早在48小时就被观察到,特别是在更高的温度 (22°C).
- 红细胞参数变化发生在4°C和8°C的48小时内,在2°C和22°C的更早时间内.
- 在冷却样本中,血小板数量随着时间的推移出乎意料地增加,从24小时开始显示出温度依赖的趋势.
结论:
- 马类血液样本在4°C或8°C保存时表现出最大的稳定性.
- 建议在采集后48小时内进行分析,以获得可靠的CBC结果,使用IDEXX Procyte Dx.
- 储存温度对各种CBC参数的完整性随着时间的推移产生重大影响.
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