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Updated: Jan 21, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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工程 GFP-NanoLuc 聚变基质用于通过 BRET 灵敏,定量检测蛋白酶活动

Mitsuki Nakamura1, Masafumi Sakono1

  • 1Department of Applied Chemistry, Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-855, Japan.

Analytical biochemistry
|January 19, 2026
PubMed
概括

研究人员开发了一种新的,基因编码的生物发光共振能量转移 (BRET) 基质,用于检测蛋白酶活性. 这种具有成本效益的系统消除了对合成基质的需求,使生物样品中的实时蛋白酶测量成为可能.

关键词:
生物发光共振传输能量转移.在Caspase-3中使用.蛋白酶活性测定试验再组合蛋白质的表达方式在 TEV 蛋白酶中,

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