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Updated: Jan 21, 2026

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开发Ogataea parapolymorpha作为一种通过全球代谢工程生产β-胡卜素的新平台
Jiaqi Miao1,2, Xiaoyi Zou1,2, Yanshan Ke1,2
1Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
Journal of agricultural and food chemistry
|January 19, 2026
概括
酵母的代谢工程创造了一个微生物细胞工厂,以高效地生产β-胡卜素 (β-胡卜素). 这种生物方法为这种有价值的食品添加剂提供了植物提取的有希望的替代方案.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物发酵 微生物发酵
背景情况:
- 贝塔胡卜素 (β-胡卜素) 是一种重要的脂溶性类化合物,作为食品添加剂使用.
- 传统的从植物中提取面临着局限性.
- 微生物合成提供了一个可持续的替代方案.
研究的目的:
- 为了设计酵母Ogataea parapolymorpha作为β-胡卜素生物合成的细胞工厂.
- 优化代谢途径,以增强前体供应和辅因子再生.
主要方法:
- 在O. parapolymorpha.中构建了β-胡卜素生物合成途径.
- 优化了美酸盐路径以增加乙-CoA和NADPH供应.
- 降低了竞争的代谢途径的调节,以引导资源向β-胡卜素生产.
- 在5L生物反应器中进行料批发发酵.
主要成果:
- 达到3861.21 mg/L的高β-胡卜素标位.
- 在O. parapolymorpha.中证明了全球代谢工程的有效性.
- 建立了一个强大的酵母平台,用于胡卜素生物合成.
结论:
- O. parapolymorpha 的代谢工程为β-胡卜素生产提供了一个有效的平台.
- 这种微生物方法为植物性提取方法提供了可行的替代方案.
- 这项研究促进了有价值的胡卜素的生物合成.
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