通过调节MAPK信号传递和代谢途径来调节Bactrocera dorsalis (Diptera: Tephritidae) 的寒冷适应机制
Ghulam Murtaza1, Farman Ullah2, Zihua Zhao1
1State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Journal of insect science (Online)
|January 20, 2026
概括
东方果 (Bactrocera dorsalis) 通过在特定的组织中调整其基因而在寒冷中生存. 通过RNAi向Ty3-I和Ty3-G转体素显示出控制这种入侵性害虫的希望.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 虫害管理 虫害管理 虫害管理
背景情况:
- 巴克特罗塞拉dorsalis是一种主要的入侵性害虫,具有重大农业影响.
- 了解其寒冷应激适应对于有效的害虫控制策略至关重要.
研究的目的:
- 为了研究 Bactrocera dorsalis 对寒冷压力的组织特异性分子和生存反应.
- 为了确定关键的基因和途径涉及寒冷耐受性潜在的害虫防治应用.
主要方法:
- 转录组分析以识别在寒冷压力下头部和脂肪体中的差异表达基因.
- 基因和基因组的京都百科全书 (KEGG) 途径分析,以了解激活的分子途径.
- RNA干扰 (RNAi) 用于击败特定的转子子基因 (Ty3-I和Ty3-G) 并评估它们对幼虫存活的影响.
主要成果:
- 寒冷压力显著影响了Bactrocera dorsalis的生存率.
- 在寒冷压力下,头部和脂肪体的信号和代谢途径被激活.
- 184和365个基因分别在头部和脂肪体中被差异地表达.
- 对Ty3-I和Ty3-GGGag-Pol多蛋白质的破坏显著降低了幼虫的存活率.
- 在寒冷压力下,Ty3-I和Ty3-G的表达在头部减少,但在脂肪体中没有显著减少,这表明有组织特异性反应.
结论:
- Ty3-I和Ty3-G Gag-Pol多蛋白质在Bactrocera dorsalis寒冷应激反应和生存中起着组织特异性的作用.
- 鉴定出的应激反应基因为开发基于RNAi的害虫控制策略提供了基础,这些策略针对应激适应所必需的基因通路.
相关概念视频
01:07MAPK Signaling Cascades
8.0K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.0K
01:19Regulation of Metabolism
11.4K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.4K
02:45Responses to Heat and Cold Stress
14.7K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.7K
01:19Interactions Between Signaling Pathways
7.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.2K
03:14Notch Signaling Pathway
6.5K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.5K
02:33Hedgehog Signaling Pathway
9.8K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.8K