基化黄金纳米粒子作为抗体查测试的新型聚合增强介质
Siti Salmah Noordin1, Nordiyana Ishak2, Muhammad Azrul Zabidi3
1Department of Clinical Medicine, Advanced Medical and Dental Institute, University Sains, Kepala Batas, Malaysia.
概括
聚乙烯糖醇 (PEG) 结合金纳米颗粒 (AuNPs) 在抗体查测试 (AST) 中显著增强红细胞聚合. 与低离子强度溶液 (LISS) 相比,这种新型介质可以改善临床显著的配对抗体的检测.
科学领域:
- 生物技术是生物技术.
- 纳米技术 纳米技术
- 免疫学 免疫学 免疫学
背景情况:
- 目前的抗体查测试 (AST) 在检测临床意义上的所有抗体方面存在局限性.
- 低离子强度溶液 (LISS) 是一种常用的增强介质,但具有低于最佳的灵敏度.
- 需要新的增强介质来改善输血诊断中的alloantibody检测.
研究的目的:
- 开发和评估聚乙烯糖醇 (PEG) 结合金纳米颗粒 (AuNPs) 作为一种新的AST增强介质.
- 为了比较PEG-AuNPs与低离子强度溶液 (LISS) 在检测alloantibodies方面的性能.
- 评估PEG-AuNPs作为抗体查的改进替代品的潜力.
主要方法:
- 合成PEG结合的AuNP使用一步式方法.
- 使用紫外线可见光谱学,电子显微镜 (EFTEM,FESEM),粒子大小分析,泽塔电位测量和FT-IR光谱学来表征PEG-AuNP.
- 在120个血样本中评估PEG-AuNPs与LISS的性能,通过在抗人类球蛋白 (AHG) 阶段得分红细胞 (RBC) 凝结反应.
主要成果:
- 合成的PEG-AuNP在529nm时表现出特有的表面等离子体共振,并表现出良好的稳定性.
- 与LISS相比,PEG-AuNPs在AHG阶段显示了显著更高的红细胞凝结分数 (p=0.010).
- 对抗-D (p=0.007) 和联合抗-Lea/抗-Leb抗体 (p=0.036) 的增强凝结特别值得注意.
结论:
- 在抗体查测试中,PEG结合的AuNP显著增强了红细胞结合反应.
- 这种新型增强剂介质可以更好地检测特定的临床显著的alloantibodies.
- PEG-AuNPs代表了对LISS的有希望的替代品,可以提高AST的灵敏度和有效性.
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