相关实验视频

Updated: Jan 22, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

化学探测策略的最新进展,用于在vivo中确定RNA结构

Maryana Yarshova¹, Jieyu Zhao¹, Chun Kit Kwok^1,2

¹Department of Chemistry and State Key Laboratory of Marine Environmental Health, City University of Hong Kong, Hong Kong SAR, China.

Chemistry (Weinheim an der Bergstrasse, Germany)

|January 20, 2026

概括

研究细胞中的RNA结构是具有挑战性的. 新的化学探测方法和测序技术现在揭示了RNA结构体在其原生细胞环境中.

科学领域:

分子生物学分子生物学
生物化学生物化学
基因组学就是基因组学.

背景情况:

细胞RNA分子具有复杂的结构,对它们的功能至关重要.
由于in silico和in vitro方法的局限性,研究本地细胞环境中的RNA结构是很困难的.
细胞环境显著影响RNA结构,这是传统实验设置中经常缺失的因素.

研究的目的:

审查RNA结构探测技术的进步,以研究RNA在其原生细胞环境中.
突出新型化学探针和用于RNA结构分析的 adduct检测方法.
讨论这些方法的应用,以了解单分子和单细胞水平的RNA结构.

主要方法:

开发用于RNA结构映射的细胞透性化学探针.
整合RNA结构探测与第二代和第三代测序技术.
应用先进的 adduct检测技术用于高分辨率的RNA结构确定.

主要成果:

通过RNA结构探测,可以在体内捕获RNA结构格局.
先进的测序和检测方法促进了对RNA结构的深入分析.
这些技术允许在单分子,单细胞和亚细胞水平上检查RNA结构.

结论:

与测序相结合的RNA结构探测代表了研究功能性RNA结构的强大方法.
未来的方向包括为独特的生物问题开发更具创新性的方法.
这一领域为RNA生物发生,调节和细胞内的功能提供了深刻的见解.

关键词:

结构 RNA 结构 RNA 结构 RNA-RNA 相互作用化学探测化学探测化学探测核酸是核酸中的一种.结构的确定结构的确定

更多相关视频

RNA Secondary Structure Prediction Using High-throughput SHAPE

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

相关概念视频

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

Experimental Determination of Chemical Formula

Experimental Determination of Chemical Formula

The elemental makeup of a compound defines its chemical identity, and chemical formulas are the most concise way of representing this elemental makeup. When a compound’s formula is unknown, measuring the mass of its constituent elements is often the first step in determining the formula experimentally.

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles

Chemical Stoichiometry and Gases: Using Ideal Gas Law to Determine Moles

Chemical stoichiometry describes the quantitative relationships between reactants and products in chemical reactions.

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...