通过LC-MS/MS同时确定绿色咖啡豆中的草甘,氨基甲基酸和葡萄糖酸:优化,验证和现场研究
Júlio César R M da Silva1, Millena Christie F Avelar2, Márcia C M Ribeiro2
1Department of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
Journal of agricultural and food chemistry
|January 20, 2026
概括
一种新的,具有成本效益的方法可以在绿色咖啡豆中检测到草甘和相关的除草剂. 植物的高度影响残留物水平,对国际咖啡贸易法规和安全至关重要.
科学领域:
- 农业化学 农业化学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 草甘在咖啡种植中被广泛使用.
- 现有的检测咖啡中的除草剂的方法复杂且昂贵.
- 绿色咖啡豆是主要的出口形式,并受国际法规的约束.
研究的目的:
- 开发和验证一种简单,低成本的方法来检测绿色咖啡豆中的草甘,AMPA和葡萄糖酸盐.
- 为这些除草剂建立可靠的量化极限.
- 应用该方法来评估不同咖啡品种的残留量.
主要方法:
- 优化了一种液体染色学-并联质谱法 (LC-MS/MS) 方法.
- 使用了经过修改的QuPPe-PO提取方法,然后进行液体-液体和分散型固体相提取清理.
- 根据SANTE/11312/2021指南验证了该方法.
主要成果:
- 达到量化的低极限:0.02毫克公斤-1 (草甘),0.04毫克公斤-1 (AMPA) 和0.01毫克公斤-1 (葡萄糖酸盐).
- 方法验证证实了线性,精度和准确性.
- 应用于阿拉伯咖啡和康尼隆咖啡,确定植物高度是影响残留物水平的关键因素.
结论:
- 开发的LC-MS/MS方法可靠且具有成本效益,用于监测绿色咖啡中的极地除草剂.
- 该方法不需要衍生,简化了分析.
- 这些发现支持咖啡行业的监管合规性和食品安全性.
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