一种基于高产率的核心--上转换纳米粒子侧向流免疫试验,用于敏感检测心脏特罗素I
Zichen Wang1, Wei Zhou1, Yue Cui2
1School of Materials Science and Engineering，State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing, 100871, People's Republic of China.
Analytical and bioanalytical chemistry
|January 20, 2026
概括
我们开发了新的核心--升转换纳米粒子 (UCNPs),用于高度敏感的光侧流免疫试验 (FLFIA). 这一进步改善了临床诊断的生物标志物检测,提供了精确的结果,减少了干扰.
科学领域:
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 上转化纳米粒子 (UCNPs) 在临床诊断中为光侧流免疫试验 (FLFIA) 提供了潜力.
- 目前的FLFIA方法在检测低度生物标志物的灵敏度方面存在问题.
- 自体光干扰也会影响诊断的准确性.
研究的目的:
- 开发一种新的核心--UCNP探测器,以提高FLFIA的灵敏度.
- 创建一个基于UCNP的FLFIA平台,用于敏感地检测心脏素I (cTnI).
- 评估UCNP-FLFIA系统在缓冲,血清和临床样本中的性能.
主要方法:
- 合成的NaLuF4@NaLuF4:Yb,Er@NaLuF4 核心--UCNPs. 这是一个非常简单的方法.
- 开发了一种FLFIA试验,使用UCNP作为cTnI检测的探针.
- 将FLFIA与便携式智能手机平台集成,用于现场分析.
- 使用缓冲剂,血清和临床样本验证了试验.
主要成果:
- 核心外UCNP的光强度是传统核心外UCNP的2.4倍.
- 达到0.035 ng/mL (缓冲) 和0.059 ng/mL (血清) 的低检测极限,用于cTnI.
- 在临床样本测试中证明了高准确性和与医院结果的良好相关性.
- 在UCNP-FLFIA显示了对自身光干扰的抗性.
结论:
- 新的NaLuF4@NaLuF4:Yb,Er@NaLuF4 UCNPs显著提高了FLFIA的灵敏度和准确性.
- 基于UCNP的FLFIA平台能够快速,现场和可靠地在护理中心检测cTnI等生物标志物.
- 这项技术有望改善早期临床诊断和患者管理.
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