指蛋白ZRANB2的化调节其RNA结合和替代拼接功能
Matthew S Hursey1, Abigail D Reitz1, Samuel E Fidler1
1Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD 21201-1180, USA.
Journal of inorganic biochemistry
|January 20, 2026
概括
透硫化,是一种硫化 (H2S) 的修饰,调节RNA结合蛋白ZRANB2.2. 这一过程破坏了指 (ZF) 的结构和功能,影响了RNA结合和替代拼接.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 翻译后修改 翻译后修改
背景情况:
- ZRANB2是一种RNA结合蛋白,对于替代拼接至关重要.
- 它的功能依赖于由Zn (II) 协调的指 (ZF) 域.
- 透硫化,是一种硫化 (H2S) 的修饰,是ZF蛋白的已知调节剂.
研究的目的:
- 研究由H2S直接修改ZRANB2的情况.
- 了解固硫化如何影响ZRANB2结构,RNA结合和拼接.
- 探索透硫化作为ZF蛋白的调节机制的潜力.
主要方法:
- 复合表达ZRANB2两个域结构 (ZRANB2-2D).
- 将ZRANB2-2D暴露在H2S和氧气中以诱导持续硫化.
- 使用生物物理方法评估结构变化.
- 使用特定RNA寡核酸的RNA结合测试.
- 在体外和细胞测试以评估拼接活动.
- 减少治疗以评估可逆性.
主要成果:
- 在暴露于H2S和氧气时,ZRANB2-2D与Zn (II) 结合的ZRANB2-2D经历持续硫化.
- 过硫化导致Zn(II) 依赖结构的丧失和RNA结合的废止.
- 细胞H2S处理降低了TRA2B剪接,与ZRANB2再硫化相关.
- 在试验室中,在添加减少剂后,RNA结合活性被恢复.
结论:
- 透硫直接改变ZRANB2,影响其结构和RNA结合能力.
- 这种修改在调节ZRANB2介导的TRA2B的替代拼接中发挥着作用.
- 透硫化作为一种静电剂,调节ZF蛋白的功能,并提供一种新的调节机制.
相关概念视频
Alternative RNA Splicing
24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing
4.9K
4.9K
RNA Splicing
60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Chromatin Structure and RNA Splicing
3.3K
3.3K
RNA Polymerase II Accessory Proteins
10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K
Pre-mRNA Processing: RNA Splicing
6.7K
6.7K


