使用乳清蛋白分离物-德克斯联体增强封装益生菌的活力:德克斯分子质量的作用
P A Loyeau1, M L Spotti1, V Castel1
1Área de estudios Fisicoquímicos de Alimentos, Instituto de Tecnología de Alimentos (ITA), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, 3000, Argentina.
International journal of biological macromolecules
|January 20, 2026
概括
乳清蛋白分离物 (WPI) 和德克斯 (DX) 结合剂改善了微囊稳定性,用于封装乳酸细菌益生菌. 与原生WPI相比,这些WPI/DX合物在储存期间提供了更好的保护,特别是在较低的温度下.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 封装在食品加工过程中保护敏感化合物和微生物.
- 像Bifidobacterium animalis亚种这样的乳酸细菌 (LAB). 乳酸INL1和乳酸植物菌73a是潜在的益生菌,需要采取保护策略.
- 乳清蛋白分离物 (WPI) 和 (DX) 的梅拉德反应合物被探索为微封装的新型壁材料.
研究的目的:
- 为了研究 Maillard 反应 WPI 和 DX 结合物的有效性,作为封装两种乳酸细菌菌株的壁材料.
- 评估不同分子量 (6kDa和100kDa) 的德克斯对乳液稳定性和微囊性质的影响.
- 评估WPI/DX结合物在喷雾干燥和随后储存期间对细菌活性的保护作用.
主要方法:
- 通过受控加热制备WPI/DX合物 (WPI/DX6和WPI/DX100).
- 使用甘露油作为油相和合物作为封装剂的固体在油中的水乳液的配方.
- 喷雾干燥乳液以生产含有乳酸细菌 (B. animalis INL1,L. plantarum 73a) 的粉状微囊.
- 评估乳液稳定性 (液滴大小,化指数,界面活性) 和包装后和在不同温度 (-18°C,4°C,21°C) 储存期间的细菌活力.
主要成果:
- 与原生WPI相比,WPI/DX合物形成的乳液显著更稳定,滴滴尺寸更小,界面活性更高.
- 通过对 WPI 的结构性改变,WPI/DX6 提高了乳液稳定性,而 WPI/DX100 提高了两性行为和固态稳定性.
- 喷雾干燥减少了细菌细胞数量,但与原生WPI相比,WPI/DX合物在储存期间提供了更好的保护.
- 细菌活力在较低的储存温度下保存得最好,含有L. plantarum 73a的WPI/DX100微囊在4°C时显示出最高的生存率.
结论:
- WPI和DX的梅拉德反应合物是封装乳酸细菌的有效壁材料,增强了乳液稳定性.
- 德克斯特兰的分子量影响了乳液稳定机制和合物的保护性质.
- WPI/DX结合物提高了益生菌的稳定性和保质期,在较低的储存温度下获得最佳保护.
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