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通过使用光生物传感器Queen进行聚基基酸盐生产工程的Escherichia coli单细胞ATP监测

Wataru Fuji¹, Ayaka Kajikawa¹, Shin-Ichi Hachisuka²

¹Graduate School of Chemical Sciences and Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.

Journal of bioscience and bioengineering

|January 20, 2026

概括

工程化埃舍里希亚大肠杆菌产生了聚3-基酸盐 (P(3HB)) 在表达光ATP生物传感器的同时. 细胞内ATP水平在P(3HB) 合成期间保持稳定,这表明能量恒常.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
微生物工程微生物工程

背景情况:

聚酸酸 (PHAs) 是微生物聚烯,具有多种应用.
在PHA合成期间监测细胞内条件对于优化至关重要.
大肠杆菌是代谢工程的常见宿主.

研究的目的:

为了设计大肠杆菌用于聚3-基酸) [P(3HB) ]生产.
使用光生物传感器在P(3HB) 合成期间实地监测细胞内ATP水平.
在PHA生产过程中调查大肠杆菌中的能量代谢恒温.

主要方法:

在大肠杆菌中,PHA生物合成基因和 QUEEN光生物传感器的同时表达.
通过前体补充增强P(3HB) 合成的代谢途径工程.
PHA生物合成基因的构成性表达.
微观分析使用尼罗河蓝色染色来量化PHA含量.
使用 QUEEN生物传感器测量单细胞光,以评估ATP水平.

主要成果:

成功诱导在工程化大肠杆菌中产生P(3HB).
78%的尼罗河蓝染色细胞显示PHA含有.
皇后生物传感器在生产和非生产条件下都得到了功能表达.
在生产P(3HB) 细胞和非生产细胞之间没有观察到细胞内ATP水平的显著差异.
皇后光精确地反映了细胞内ATP水平.

结论:

大肠杆菌可以被设计为高效的P(3HB) 生产.
女王生物传感器是监测单个微生物细胞中的ATP水平的可靠工具.
大肠杆菌在P(3HB) 合成过程中表现出强大的能量代谢平衡.
这项研究提供了关于工程细菌中PHA生产的代谢调节的见解.

关键词:

在ATP的同居状态下,ATP的同居状态.基于生物的塑料.光蛋白生物传感器是一种生物传感器.聚氨酸多基甲基酸电比度生物传感器

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