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Updated: Jan 22, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
Published on: August 29, 2025
无标签组织NIR-II自光成像用于可视化人类肝脏恶性瘤
Haisheng He1, Wenwei Zhu2, Han Miao3
1Department of Chemistry, College of Smart Materials and Future Energy, School of Pharmaceutical Sciences, New Cornerstone Science Laboratory, State Key Laboratory of Molecular Engineering of Polymers and iChem, Key Laboratory of Smart Drug Delivery Ministry of Education and State Key Laboratory of Advanced Drug Formulations for Overcoming Delivery Barriers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Wusong Laboratory of Materials Science, Fudan University, Shanghai Academy of Natural Sciences (SANS), Shanghai, China.
研究人员开发了组织自光近红外II成像 (TANI) 来检测肝癌. 这种无标签的方法使用自然的自发光来精确地划定手术期间的瘤边界.
科学领域:
- 生物医学光学 生物医学光学
- 手术瘤学手术瘤学
- 医疗成像医学成像
背景情况:
- 准确的手术内瘤边界划定对于成功的手术切除至关重要,但仍然是一个临床挑战.
- 现有的成像工具往往缺乏复杂案例所需的实时精度.
研究的目的:
- 开发和验证一种新的无标签成像技术,用于实时可视化人类肝脏恶性瘤.
- 在第二个近红外窗口 (NIR-II) 中利用内源性自光,以增强瘤和健康组织之间的对比度.
主要方法:
- 在NIR-II窗口 (1,000-1,700 nm) 中识别具有强烈自光的内源物质,存在于肝脏组织中,但不在瘤中.
- 组织自光NIR-II成像 (TANI) 的开发,是一种用于可视化肝脏恶性瘤的广场成像方法.
- 与传统成像和光导向手术相比,TANI性能的比较分析.
主要成果:
- 在划分各种肝脏恶性瘤方面,TANI获得了高对比度 (7.69 ± 0.52),灵敏度 (97.8%) 和特异性 (98.4%).
- 该技术准确地可视化了肝细胞癌,肝内胆管癌和肝转移.
- 在不同癌症等级/阶段,TANI的性能强大,不受血液/胆汁污染或良性病变的影响.
结论:
- TANI是一种高效的,无标签的成像方法,用于肝脏恶性瘤的手术内可视化.
- 这些发现表明NIR-II自光和疾病状态之间存在强烈的相关性,在手术决策方面提供了有前途的进展.
- 对这些自发光物质的分子基础的进一步研究可能会产生新的诊断策略.
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