在老鼠组织模型中比较吸烟对椎间盘细胞代谢的影响
Nathan Buchweitz1, Avery Madden1, Joshua Kelley1
1Department of Bioengineering, Clemson University, Clemson, SC, USA.
Annals of biomedical engineering
|January 20, 2026
概括
吸烟会通过直接的毒性和营养缺乏对椎间盘 (IVD) 细胞造成伤害,减少能量生产. 细胞核最受影响,而软骨末端板更有弹性.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- 椎间盘 (IVD) 退化是腰部疼痛的主要原因之一.
- 吸烟是已知的IVD退化风险因素之一.
- 吸烟影响IVD细胞代谢的确切机制尚不完全理解.
研究的目的:
- 研究吸烟对腰部IVD细胞能量代谢的直接和间接影响.
- 分析不同IVD地区的葡萄糖消耗和乳酸盐产量.
- 在吸烟相关条件下模拟营养物质运输和ATP合成.
主要方法:
- 来自老鼠的腰部IVD被剖析成细胞核 (NP),纤维环 (AF) 和软骨末端板 (CEP) 区域.
- 组织在受控,香烟烟雾提取物 (CSE) 或低营养条件下进行培养.
- 测量和建模了葡萄糖消耗率 (GCR),乳酸生产率 (LPR) 和ATP合成.
主要成果:
- 在CSE和低营养条件下,AF和NP的GCR和LPR显著减少.
- 低营养条件增加了乳酸盐生产率与葡萄糖消耗率的比率,这表明糖解的增加.
- 计算建模显示,CSE减少了ATP合成,而营养缺乏的条件严重耗尽了葡萄糖,氧气和ATP,综合效应更糟.
结论:
- 吸烟会通过直接的毒性和间接的营养缺乏影响IVD细胞能量代谢.
- 细胞核 (NP) 是代谢上最敏感的区域,而软骨末端板 (CEP) 则更有弹性.
- 这些发现为IVD对吸烟的代谢适应及其在退化中的作用提供了洞察力.
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