从血素和氨酸染色图像的单细胞形态测量揭示了慢性淋巴细胞白血病中亚型特征
María Cecilia Cabral Lorenzo1, Juan Quarroz Braghini1, Gregorio Cordini2
1División Anatomía Patológica, Hospital de Clínicas "José de San Martín", Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
来自常规活检的高维单细胞形态学可以客观地区分攻击性和惰性的慢性淋巴细胞白血病 (CLL) 病例. 这种新的方法提供了可量化的见解,以补充当前的诊断标准,以改善患者分层.
科学领域:
- 血液病理学 血液病理学
- 计算生物学 计算生物学
- 在瘤学瘤学.
背景情况:
- 慢性淋巴细胞白血病 (CLL) 是一种异质的B细胞恶性瘤,具有不同的临床攻击性.
- 目前加速性CLL (A-CLL) 的诊断标准依赖于主观组织学评估.
- 需要客观的方法来补充现有的准确患者分层标准.
研究的目的:
- 为了研究从常规H&E染色淋巴结活检中高维单细胞形态学的实用性,用于对CLL病例进行分类.
- 开发和实施一个新的,开源的计算管道,用于单细胞形态分析.
- 评估形态学数据是否可以客观地区分惰性和攻击性CLL亚型.
主要方法:
- 使用CellProfiler和Seurat进行细胞细分和参数测量的新开源方法的实施.
- 数据集的整合以减轻技术批量效应.
- 无监督和监督机器学习模型的应用,包括轨迹分析和病理学家指导的分类.
主要成果:
- 无监督和监督的形态测量分析都成功地区分了攻击性与惰性CLL病例.
- 积极的病例显示在对应于准免疫细胞的形态学集中的丰富.
- 沉默病例在代表小淋巴细胞的集群中得到了丰富,伪批量分析也取得了成功的患者分类.
结论:
- 高维单细胞形态测量提供客观,可量化的指标,反映CLL疾病连续性.
- 这种计算方法可以在诊断和分层CLL患者时补充主观组织学标准.
- 需要在更大的队列中进一步验证,以将这种方法整合到临床实践中.
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