在公羊中提高解后的精子质量:酸作为天然抗氧化剂
Barış Denk1, Murat Kırıkkulak2, Şükrü Güngör3
1Department of Biochemistry Afyonkarahisar, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye.
Frontiers in veterinary science
|January 21, 2026
概括
酸 (QA) 补充剂在解后改善公羊精液质量,增强运动性和DNA完整性. 100微克/毫升的最佳度通过减少氧化应激来保护精子.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 雄羊精液的冷保存对于人工授精计划至关重要.
- 由于氧化应激和DNA损伤,在解后保持精子质量具有挑战性.
- 酸 (QA) 正在研究其潜在的抗氧化和冷保护性质.
研究的目的:
- 为了评估不同度的酸 (QA) 对雄羊精液解后质量的影响.
- 评估QA对精子运动,DNA完整性,流细胞计参数和氧化状态的影响.
- 为了确定最佳的质量保证度,以改善雄羊精液冷保存.
主要方法:
- 雄羊精液射精被冷保存,使用基于Tris的扩展剂,含有不同的QA度 (控制,50,100,200微克/毫升).
- 用计算机辅助精子分析 (CASA),流动细胞计 (活力,线粒体活性,脂质过氧化) 和用于DNA完整性的COMET试验来评估精子质量.
- 氧化状态通过总抗氧化状态 (TAS),总氧化状态 (TOS),马隆迪化物 (MDA) 和氧化应激指数 (OSI) 来衡量.
主要成果:
- 酸在100μg/mL的水平显著改善了总和渐进性运动性和动力学参数.
- 流细胞计显示了更高的活力和线粒体膜潜力,在100μg/mLQA时降低了脂质过氧化.
- 在COMET测定中,100μg/mL的QA显示DNA碎片化减少,而TAS增强,OSI降低.
- 200微克/毫升的QA剂量表现出潜在的亲氧化作用,增加MDA和TOS.
结论:
- 酸在冷保存期间对雄羊精子质量表现出剂量依赖的保护作用.
- 100微克/毫升QA的最佳度有效减轻氧化应激,并增强解后的精液参数.
- 作为一种抗氧化剂和冷保护剂的添加剂,QA显示出改善雄羊人工授精成功的前景.
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