改制自动维生素D试验,其前身和2种当代配方的比较
K Aaron Geno1,2, Yasel F Alvarez1,2, Jacqueline A Hubbard3
1Department of Pathology and Laboratory Medicine, Dartmouth Health, Lebanon, NH, United States.
The journal of applied laboratory medicine
|January 21, 2026
概括
新的自动化25-基维生素D (25OHD) 测定显示了25OHD2的提高准确性,解决了历史恢复问题. 这些更新的测定方法适合常规临床使用,即使是服用高剂量维生素D2补充剂的患者.
科学领域:
- 临床化学 临床化学
- 生物化学测试 生物化学测试
- 维生素D的代谢过程
背景情况:
- 从历史上看,25-基维生素D (25OHD) 的测试显示了25-基维生素D2 (25OHD2) 的恢复变异性.
- 自那以后,测试制造商更新了试剂以提高准确性.
- 这项研究评估了新的自动化测试与黄金标准.
研究的目的:
- 为了比较当前自动化25OHD测试的性能.
- 在更新的测定中评估25OHD2回收的准确性.
- 为了验证试验对临床维生素D状态分类的适用性.
主要方法:
- 将第二代和第三代罗氏试验与迪亚索林和贝克曼试验进行了比较.
- 利用了75个血清样本,包括那些来自高剂量维生素D2的人.
- 根据25OHD2和25-氧维生素D3度的液体染色学-并联质谱学 (LC-MS/MS) 验证.
主要成果:
- 自动化试验的平均偏差为≤5.1 ng/mL,而LC-MS/MS则为≤5.1 ng/mL.
- 第三代罗氏试验显示25OHD2恢复的显著改善.
- 大多数自动化测试表明,与LC-MS/MS有充分的临床分类协议.
结论:
- 自动化25OHD测定有了显著的改进.
- 25OHD2回收不再是评估试验的主要关注点.
- 所有测试的试剂都适合用于维生素D状态的常规临床分类.
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