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Updated: Jan 22, 2026

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

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Published on: August 12, 2021

一个基于AND逻辑门的生物传感器,用于同时检测SARS-CoV-2核酸和核体蛋白

Zhongzhong Wan^1,2, Chengchen Xu¹, Yuchao Wang^2,3

¹Institute of Biology and Medicine, College of Life Science and Health, Wuhan University of Science and Technology, Wuhan 430065, P. R. China.

Analytical chemistry

|January 21, 2026

概括

这项研究引入了一种新的生物传感器,可同时检测SARS-CoV-2RNA和核囊蛋白. 一同热放大和CRISPR-Cas合作系统 (OIACS) 通过减少假阳性来提高诊断准确性.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
生物传感器技术技术

背景情况:

核酸和蛋白质是疾病诊断的关键生物标志物.
传统的单分析检测方法容易产生假阳性,影响可靠性.
目前的方法在同时检测多个生物标志物,如病毒RNA和蛋白质时面临挑战.

研究的目的:

开发一种新的生物传感器,同时检测SARS-CoV-2RNA和核囊蛋白.
通过 AND 逻辑门方法,通过最小化错误阳性来提高诊断可靠性.
为了创建一个灵活的测试设计,用于多样化的目标检测.

主要方法:

开发了一个单异热放大和CRISPR-Cas合作系统 (OIACS).
利用抗体介导的标结合与阻断剂释放用于灵活的标识别.
通过链位移异热放大来进行信号放大,生成了通用Cas12a可定位DNA条形码.

主要成果:

该OIACS功能作为一个AND逻辑门生物传感器,用于同时检测.
在5000个副本/毫升的情况下,实现了SARS-CoV-2病毒样颗粒的可靠检测.
确定的检测极限低至1698个副本/mL.

结论:

OIACS提供了一种强大而可靠的双生物标志物检测方法.
证明了SARS-CoV-2临床诊断的实际实用性和潜力.
抗体介导识别和DNA条形码放大提高了测试性能.

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