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Updated: Jan 22, 2026

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A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
Published on: June 26, 2014
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在猪中实验Salmonella Typhimurium感染
Gabriel A de Aguiar1, Daniela G da Silva1, Laíza P Arruda1
1School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, Brazil.
概括
在猪中实验性感染沙门氏菌Typhimurium导致腹和特定的肠道病变. 有趣的是,在没有临床疾病迹象的猪身上检测到沙门氏菌.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 动物科学动物科学
背景情况:
- 沙门氏菌Typhimurium感染对猪的健康和经济可行性构成重大威胁.
- 猪中沙门氏菌Typhimurium的临床表现和病理结果需要进一步阐明.
研究的目的:
- 调查猪实验性沙门氏菌Typhimurium感染的临床和病理影响.
- 为了将沙门氏菌Typhimurium脱落与临床症状和死后发现相关联.
主要方法:
- 20只90天大的猪被试验性地用沙门氏菌Typhimurium (G1) 或安慰剂 (G2) 接种.
- 对临床症状,直肠温度和体重进行了30天的监测.
- 进行了死后检查和微生物学分析,以评估病变和细菌脱落.
主要成果:
- 感染沙门氏菌Typhimurium的猪从注射后的第五天开始出现腹.
- 在便样本中间歇检测到沙门氏菌Typhimurium,主要来自没有腹的猪.
- 宏观性病变包括大肠头,中肠淋巴结扩大,以及在受挑战的猪中结肠淋巴细胞增生.
结论:
- 猪中的沙门氏菌 Typhimurium 感染会导致明显的临床症状和病理变化.
- 即使在没有腹的情况下,沙门氏菌 Typhimurium 的间歇性泄漏也突出了疾病传播的复杂性.
- 这些发现强调了全面监测和诊断策略对于控制猪群中的沙门氏菌的重要性.
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