针对SARS-CoV-2核体蛋白的抗体具有自身免疫性质
Alexandra Rak1, Yana Zabrodskaya2, Pei-Fong Wong1
1Depatment of Virology and Immunology, Institute of Experimental Medicine, 197022 Saint Petersburg, Russia.
Antibodies (Basel, Switzerland)
|January 21, 2026
概括
调查SARS-CoV-2核体蛋白 (N) 交叉反应性揭示了潜在的自身免疫点,如小鼠和子的热冲击蛋白和组织蛋白. 这一发现对于理解COVID后的状况和设计未来疫苗至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 疫苗开发 疫苗开发
背景情况:
- 尽管COVID-19大流行正式结束,但SARS-CoV-2仍在继续引起疾病和长期的COVID后状况,可能是由于自身免疫反应.
- SARS-CoV-2的保存和免疫性核体 (N) 蛋白质是疫苗开发的目标,但其在抗体诱导的自身免疫性中的作用需要进一步研究.
- 了解抗N抗体和人类抗原之间的交叉反应性对于预测和减轻与自然感染或基于N的疫苗相关的自身免疫风险至关重要.
研究的目的:
- 为了确定SARS-CoV-2 N蛋白和人类抗原之间的潜在结构或序列同质性.
- 研究高免疫血清对N蛋白与人类自身抗原的交叉反应性.
- 评估抗N抗体的免疫性和潜在的自身免疫影响.
主要方法:
- 对人类细胞系溶解物的西部斑分析,以检测抗体交叉反应性.
- 通过抗N抗体识别人类自我抗原的质谱识别.
- 在小鼠,子和仓鼠中对抗N抗体反应的比较.
主要成果:
- 来自小鼠和子的抗N抗体显示出与人类蛋白质的显著交叉反应,包括热冲击蛋白 (HSP90-β,HSP70,HSP60,HSPA8),基因组 (H2B,H3.1-3) 和代谢酶 (G6PD,GP3,PKM).
- 来自子的Anti-N免疫球蛋白与人类或子蛋白质基提取物没有交叉反应,这表明该模型中缺乏自身活性.
- 质谱测量确定了特定的自身抗原标,主要是热冲击蛋白和基因组.
结论:
- 对SARS-CoV-2 N蛋白质的幽默性免疫反应在物种之间存在显著差异,特别是在人类和叙利亚仓鼠模型之间.
- 鉴定的交叉反应抗原为预测COVID-19康复者潜在的自身免疫后果提供了基础.
- 这些发现对于未来基于N蛋白质的疫苗的合理设计至关重要,为新型冠状病毒提供交叉保护.
相关概念视频
Antibody Structure
65.4K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.4K
Autoimmune Disorders
1.5K
Autoimmune diseases are a group of disorders in which the body's immune system mistakenly attacks its own cells, tissues, and organs. This results from an overactive immune response against substances and tissues normally present in the body. Let's delve into the concept and mechanism of autoimmune diseases from an immune system point of view, explore different causes and examples of such diseases, and discuss potential solutions.
Concept and Mechanism of Autoimmune Diseases
The immune...
Concept and Mechanism of Autoimmune Diseases
The immune...
1.5K
Properties of Transition Metals
29.6K
Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
29.6K
Physical and Chemical Properties of Matter
165.5K
The characteristics that enable us to distinguish one substance from another are called properties.
165.5K
Protein-protein Interfaces
14.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.5K
Introduction to Membrane Proteins
80.7K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
80.7K


