对羊群PAPPA2表达和遗传变异的全面分析以及它们与子大小的关联
Yuanyuan Wang1, Wenqiang Xu1,2, Benmeng Liang2
1School of Life Science, Bengbu Medical University, Bengbu, People's Republic of China.
Veterinary medicine and science
|January 21, 2026
概括
一种特定的PAPPA2基因变异显著影响绵羊子的大小,为选择性繁殖提供了改善绵羊生殖特征的潜力.
科学领域:
- 动物遗传学动物遗传学
- 生殖生物学 生殖生物学
- 基因组选择 基因组选择
背景情况:
- PAPPA2基因对于调节胰岛素样生长因子 (IGF) 的生物可用性至关重要.
- 它在羊的繁殖特征,特别是子大小中的作用尚不清楚.
- IGFBP5被PAPPA2分裂影响生殖功能.
研究的目的:
- 调查羊群中PAPPA2基因表达模式.
- 确定与 litter 尺寸相关的 PAPPA2 基因多态.
- 分析已识别的多形态对PAPPA2蛋白的结构影响.
主要方法:
- 来自六个品种的760只母羊的基因型为两个PAPPA2SNP (g.55757202C>A和g.55924884C>G).
- 进行定量PCR (qPCR) 进行组织特异性基因表达分析.
- 利用生物信息学工具来评估突变的结构后果.
主要成果:
- g.55924884C>G SNP与小尾汉羊的子大小有显著的相关性.
- 与高生育率的小尾汉羊相比,在低生育率的西藏羊的卵巢中,PAPPA2的表达显著更高.
- 这种g.55924884C>G突变导致了PAPPA2蛋白的显著结构变化.
结论:
- PAPPA2基因中的g.55924884C>G多态性是影响绵羊子大小的关键因素.
- 这种特定的多态可能作为一种有价值的遗传标记,用于选择性养计划在绵羊.
- 了解PAPPA2的作用可以提高绵羊的繁殖效率.
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