相关实验视频
Updated: Jan 22, 2026
13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
9.3K
爆炸抗性基因Pish衍生了大米中非编码RNA的重编程
Lina Liu1,2, Owais Iqbal1,3, Bingjuan Yang1,3
1State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
Rice (New York, N.Y.)
|January 21, 2026
概括
长非编码RNAs (lncRNAs) 在对Magnaporthe oryzae.米的免疫力中起着至关重要的作用. 这项研究确定了参与爆发抗性的新型lncRNAs,突出了它们与基因和miRNAs的调节网络,以改善疾病管理.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 基因组学就是基因组学.
背景情况:
- 米 (Oryza sativa) 和Magnaporthe oryzae表现出复杂的共同进化分子相互作用.
- 虽然在米芽相互作用中的蛋白质编码基因 (PCG) 被广泛研究,但长非编码RNAs (lncRNAs) 的作用在很大程度上是未知的.
研究的目的:
- 研究米中 lncRNAs 的作用,研究对Magnaporthe oryzae.天生的免疫力.
- 为了识别对爆发反应的 lncRNAs 并阐明它们在耐药大米系中的调节机制.
主要方法:
- 苗的全转录组链特异性RNA测序 (敏感的LTH和耐药的IRBLsh-S线) 在与M. oryzae注射后进行.
- 生物信息分析以确定差异表达的lncRNA,它们的共同表达网络和潜在的功能.
主要成果:
- 16.8%的确定的lncRNAs在注射后24小时 (hpi) 响应了抗药线上的爆发性感染,其中45.1%是新鲜的.
- 49个差异表达的lncRNAs与参与氧化应激反应和momilactone A生物合成的基因共同表达.
- 两个lncRNAs (XLOC_046130,XLOC_040277) 被预测为miRNAs的内源性标模仿物 (eTM),与转录因子共同表达以调节momilactone A合成.
结论:
- lncRNAs通过涉及PCG,转录因子和miRNAs的复杂网络来调节大米的先天免疫力.
- 这项研究为R基因介导的抗性机制提供了新的见解.
- 在米育种计划中,lncRNAs显示出提高抗病能力的潜力.
相关概念视频
02:30siRNA - Small Interfering RNAs
18.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.4K
02:39lncRNA - Long Non-coding RNAs
9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
02:39lncRNA - Long Non-coding RNAs
3.5K
3.5K
02:57piRNA - Piwi-interacting RNAs
7.5K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.5K
02:33Combinatorial Gene Control
9.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.5K
02:30Small interfering RNAs (siRNA)
4.3K
4.3K