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Updated: Jan 23, 2026

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使用乙醇和深层环氧溶剂从Jambolan果实中提取安托西亚宁:生物活性,细胞毒性,并作为天然染色剂应用
Pedro Victor Crescêncio de Freitas1, Carlos Eduardo de Araújo Padilha2, Marcos Dos Santos Lima3
1Biotechnology Postgraduate Program - RENORBIO, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
PloS one
|January 21, 2026
概括
深度浸泡溶剂 (DESs) 增强了从Jambolan果实中提取的安东素. 以胆化物为基础的DES与糖醇显示出优异的结果,产生具有抗氧化和酶抑制性质的强有力的天然色素,没有观察到毒性.
科学领域:
- 食品化学 食品化学
- 自然产品提取 提取自然产品
- 生物化学 生物化学
背景情况:
- 安西是有价值的天然颜料和生物活性化合物,存在于诸如博兰 (Syzygium cumini) 等水果中.
- 传统的提取方法通常使用苛刻的溶剂或效率低.
- 深度环氧化溶剂 (DES) 为天然产品提取提供了一个有前途的绿色替代品.
研究的目的:
- 为了评估基于胆化物的深度浸泡溶剂 (DESs) 在醇系统中作为辅溶剂的有效性,用于从Jambolan水果中提取安托素.
- 为了优化提取条件,包括DES类型,度和温度.
- 评估提取的安东素的功能性质和安全性.
主要方法:
- 使用乙醇从Jambolan果实中提取安托西亚宁,使用不同度的基于胆化物的DESs (xylitol和glycerol).
- 提取温度和时间的优化.
- 对抗生素产量,颜色强度,抗氧化能力和酶抑制 (α-氨酶和氨糖酶) 的分析.
- 使用HaCaT细胞进行细胞毒性评估.
- 在储存期间评估丰富酸奶中的色彩稳定性.
主要成果:
- 使用乙醇与5%的DES,获得了最高的安托西亚宁产量.
- 最佳提取发生在70°C,5%的基于胆糖醇的DES (ChG-DES),产生28.92毫克M3G/100g.
- 与单独的乙醇相比,ChG-DES提取物显示出增强的颜色强度,抗氧化能力和酶抑制活性.
- 在高达12.5mg/ml的HaCaT细胞中没有观察到细胞毒性.
- 安索亚宁提取物在储存期间在酸奶中表现出良好的色彩稳定性.
结论:
- 基于胆化物的DES,特别是ChG-DES,是有效的辅溶剂,可以增强从Jambolan果实中提取安东素的效果.
- 优化的提取过程产生具有显著功能性质 (着色剂,抗氧化剂,酶抑制剂) 和良好的安全性质的安托西亚宁.
- 用DES辅助提取为食品工业生产天然色素和功能成分提供了一个可行的战略.
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