通过糖异构酶基因沉默,增强桑黄的多糖化物生物合成
相关实验视频
Updated: Jan 23, 2026

10:18
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
24.9K
Bin Yu1, Jianzhong Yao2, Xiaole Liu1
1Bio-based Platform Chemicals Catalysis Engineering Technology Research and Development Center of Jiangsu Province, College of Life and Health, Nanjing Polytechnic Institute.
The Journal of general and applied microbiology
|January 21, 2026
概括
在Sanghuangporus baumii中抑制糖异合酶 (PGI) 基因显著增加了多糖的产量. 这一发现为提高这些有价值的药用化合物的工业产量提供了途径.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 桑胡安 (Sanghuangporus baumii) 聚糖 (SBP) 具有显著的药理价值.
- 尚不完全了解SBP的生物合成途径,这阻碍了优化生产.
- 糖异糖酶 (PGI) 是一种关键的代谢酶,假定可以调节真菌多糖化合物合成.
研究的目的:
- 为了研究糖异构酶 (PGI) 基因 (sbpgi) 在Sanghuangporus baumii.i.中的功能.
- 为了探索sbpgi在桑胡安菌 (Sanghuangporus baumii polysaccharides,SBP) 生物合成中的作用.
- 评估sbpgi静音对真菌生长,多糖生产和细胞壁组成的影响.
主要方法:
- 从S. baumii中克隆了sbpgi基因.
- 使用RNA干扰 (RNAi) 生成sbpgi沉默突变物.
- 对菌根生物质,外聚糖 (EPS) 和细胞内多糖 (IPS) 生产的分析.
- 细胞壁成分 (β-1,3-葡萄糖和基) 的量化.
- 关键多糖生物合成基因的转录分析.
主要成果:
- 沉默sbpgi减少了菌生物质约20%但增加了EPS和IPS产量,分别是约2.0倍和1.9倍.
- 抑制sbpgi降低了β-1,3-葡萄糖含量约23%,并增加了基沉积约1.7倍.
- sbpgi沉默改变了细胞壁结构和应激耐受性,并调高了关键的多糖生物合成基因 (例如,ugpg,pmm).
结论:
- 糖异糖酶 (PGI) 在桑黄菌 (Sanghuangporus baumii) 的多糖生物合成中起着关键的调节作用.
- sbpgi静音增强了外聚糖和细胞内多糖的产生,为代谢工程提供了潜力.
- 这些发现为开发用于工业应用的高产量SBP生产菌株提供了基础.
相关概念视频
Biosynthesis of Polysaccharides
577
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
577
Biosynthesis in Bacteria
587
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
587
Biosynthesis of Lipids
552
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
552
Biosynthesis of Nucleic Acids
1.0K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.0K
Energy-requiring Steps of Glycolysis
171.4K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
171.4K
Organization of Genes
73.2K
Overview
73.2K

