广泛的蛋白质水解剂在支持肠道屏障功能的 in vitro 作用
Valentina Bozzetti1,2, Rachel Brosnan3, Smriti Verna3
1Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, MA, USA. vbozzetti@mgh.harvard.edu.
Pediatric research
|January 21, 2026
概括
广泛化蛋白质 (eHP) 改善了早产婴儿的肠道屏障功能. 这项研究表明,eHP增强了肠道完整性和细胞活力,可能减少诸如死性肠球炎等并发症.
科学领域:
- 胃肠病学 胃肠病学
- 新生儿科学 新生儿科学
- 分子生物学分子生物学
背景情况:
- 对于早产婴儿来说,母亲的母乳是最佳的,它支持肠道成熟.
- 当母乳无法供应时,使用捐赠奶或专用配方等替代品.
- 牛奶衍生的广泛化蛋白 (eHP) 在婴儿营养中很常见,但它们对早产肠道模型的影响尚不清楚.
研究的目的:
- 为了研究广泛化蛋白 (eHP) 对肠道屏障功能和基因表达在早产的人类肠道表皮的模型的影响.
- 为了比较eHP与自由氨基酸 (AA) 和完整的母牛奶蛋白 (WP) 的影响.
主要方法:
- 胎儿人类肠道有机物衍生的单层被用于模型过早的肠道表皮.
- 在基线和炎症条件下 (细胞因子/细菌),单层被暴露在AA,WP或eHP中.
- 评估了屏障完整性,透性,细胞活力和基因表达 (RNA-seq).
主要成果:
- 在基线和炎症条件下,eHP显著降低了上皮膜透性.
- 在炎症期间,eHP和AA在低度下改善了细胞活力.
- 转录组分析显示eHP调节了参与扩散和修复的NOTCH和WNT信号通路.
结论:
- 在早产肠道模型中,eHP增强了肠道屏障功能和细胞活力,无论是在基线还是炎症期间.
- 这些发现支持使用eHP作为改善早产婴儿肠道完整性的营养策略.
- 需要进一步的研究来验证这些发现,并探索基于的配方的临床应用.
相关概念视频
01:21Barriers to Effective Communication II
4.6K
The barriers to effective communication also include cultural barriers, semantic barriers, gender barriers, and time constraints.
Cultural barriers:
Differences in values, beliefs, religion, knowledge, and tradition can significantly impact communication. Awareness of nonverbal cues is critical, especially when conversing with a patient from a different culture. What appears appropriate in one culture may be inappropriate in another.
Semantic barriers:
As a result of their tendency to use...
Cultural barriers:
Differences in values, beliefs, religion, knowledge, and tradition can significantly impact communication. Awareness of nonverbal cues is critical, especially when conversing with a patient from a different culture. What appears appropriate in one culture may be inappropriate in another.
Semantic barriers:
As a result of their tendency to use...
4.6K
01:30Barriers to Effective Communication I
11.0K
A communication barrier is any distortion or interruption during a conversation, resulting in miscommunication of the message. A good communicator should know these barriers and continuously check for the listener's understanding by obtaining feedback.
Communication barriers include the following:
Physiological barriers: They are limitations caused by a person's health condition or disability, such as hearing loss, poor eyesight, illness, or unconsciousness. An example to overcome this...
Communication barriers include the following:
Physiological barriers: They are limitations caused by a person's health condition or disability, such as hearing loss, poor eyesight, illness, or unconsciousness. An example to overcome this...
11.0K
01:56Structural Protein Function
29.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
29.8K
01:56Structural Protein Function
3.2K
3.2K
01:20Digestive Functions of the Large Intestine
2.1K
The large intestine is where the final stages of digestion happen. When the cecum receives chyme, it contains undigested carbohydrates that undergo fermentation. Gut bacteria ferment these carbohydrates to produce short-chain fatty acids that provide some energy and help synthesize essential vitamins.
As the chyme moves to the colon, it triggers two characteristic sluggish contractions - haustral churning and mass peristalsis. Haustral churning involves the rhythmic contraction and relaxation...
As the chyme moves to the colon, it triggers two characteristic sluggish contractions - haustral churning and mass peristalsis. Haustral churning involves the rhythmic contraction and relaxation...
2.1K
01:23Anatomy of the Intestines
87.0K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
87.0K