通过吸附方法在MnO2纳米颗粒上固定的乳酶的制备,表征和酶性质
Wanlei Yue1, Xin Wang2, Zixin Gao1
1School of Environmental and Chemical Engineering, Shenyang University of Technology, Shenyang, 110870, China.
Biotechnology letters
|January 21, 2026
概括
在δ-MnO2板上固定乳酶显著提高了其稳定性和可用于废水处理的可重复使用性. 这种新型的MnO2@Lac生物催化剂显示了酶活性的改善和广泛的应用前景.
科学领域:
- 生物催化和纳米材料科学
- 环境生物技术 环境生物技术
- 酶工程是什么? 酶工程是什么?
背景情况:
- 自由乳酸对pH和温度等环境因素具有有限的稳定性.
- 纳米材料固定化提供了一种策略,以克服乳酸的不稳定性并提高可重复使用性.
- 二氧化 (MnO2) 纳米材料被探索为酶不动化的载体.
研究的目的:
- 为了研究乳糖酶对不同MnO2形态的固定.
- 为了评估固定乳酶的增强酶性质和稳定性.
- 评估MnO2-不动化的乳酸酶 (MnO2@Lac) 在污染物处理中的潜力.
主要方法:
- 四种MnO2形态的比较,用于乳糖吸附,确定 δ-MnO2 (叶片) 是最优的.
- 优化固定条件 (pH,温度,时间,酶度).
- 使用FTIR和SEM进行表征;研究MnO2@Lac.的酶性质和稳定性.
主要成果:
- δ-MnO2被证明是对laccase最有效的固定载体.
- 在pH值为5,30°C,1毫克/升的乳糖中,在4小时内达到最佳固定.
- 静止的乳酶表现出增强的稳定性 (最佳pH值为3,75°C),并在30天后在-4°C下保持74.51%的活性.
- MnO2@Lac与自由乳酸酶相比,显示反应速度增加,基质亲和力改善,相对酶活性增强.
结论:
- δ-MnO2是乳糖酶固定化的有效载体,显著提高了酶的稳定性和活性.
- MnO2纳米粒子和乳糖酶之间的协同效应有助于提高生物催化性能.
- 在有机污染物处理中的应用中,MnO2不动化的乳具有显著的前景.
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