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开发一种新的人性化肠-大脑轴模型,作为实现个性化营养的工具

Myrto S Chatzopoulou¹, Ravi Vumma^2,3, Samira Prado^1,4

¹School of Medical Sciences, Faculty of Medicine and Health, Örebro University, 70182, Örebro, Sweden.

Communications biology

|January 21, 2026

概括

这项研究开发了一种新的肠-大脑轴模型,使用人类细胞来研究肠道微生物如何影响血脑屏障 (BBB). 结果显示,肠道液体保护BBB细胞免受氧化应激,为神经健康提供了洞察力.

科学领域:

微生物学微生物学
神经科学是一个神经科学.
胃肠病学胃肠病学

背景情况:

肠道微生物代谢物影响托和血清的代谢,影响肠-大脑轴.
现有的模型缺乏充分理解这些肠-大脑相互作用所需的整合.
血脑屏障 (BBB) 是一个由肠道衍生因素影响的关键接口.

研究的目的:

开发和验证一种ex vivo-in vitro模型系统,以研究肠道光线含量对血脑屏障 (BBB) 的直接影响.
为了研究人类结肠血清液在氧化应激下对BBB代表细胞的保护作用.

主要方法:

人体皮肤纤维细胞 (代表BBB) 用人体结肠活检中的血清液培养.
从Ussing室实验中获得了活检,这些实验涉及体内酸盐暴露,体外纤维发酵或对照超剂.
评估了纤维细胞活力,细胞毒性,托芬载体基因/蛋白质表达和托芬吸收.
纤维细胞暴露在氧化应激 (H2O2) 中,有或没有先前与血清液体进行化.

主要成果:

用血清液培养纤维细胞并没有损害细胞活力或表现出细胞毒性作用.
血清液单独没有改变托载体基因表达,蛋白质水平或托吸收.
用血清液进行预化证明了对纤维细胞的保护作用,防止氧化应激.

结论:

开发的ex vivo-in vitro模型有效地模拟了BBB水平的肠-大脑轴相互作用.
肠道血清液具有与BBB功能相关的抗氧化应激的保护性质.
这种模型系统在肠-大脑轴研究中对个性化医学应用具有前景.

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